Fragment-protein interactions

Examples of Fragment Screening 3.1 Fragment-Protein Interactions... 

The genome of the Helicobacterpylori bacterium is 1.6 million base pairs in size and encodes 1590 ORFs (Tomb et al., 1997). The comprehensive two-hybrid library screen performed with these ORFs differs from the yeast experiments described above in that the Gal4 activation domain library used consisted of over ten million random genomic fragments (Rain et al., 2001). Thus, the potential problem of full-size ORFs masking protein-protein interactions is reduced. A total of 261 ORFs were fused to the Gal4 DNA binding domain to create a set of baits. These ORFs... 

Protein fragment complementation assays are based on an enzyme reassembly strategy whereby a protein-protein interaction promotes the efficient refolding and complementation of enzyme fragments to restore an active enzyme. The approach was initially developed using the reconstitution of ubiquitin as a sensor for protein-protein interactions (Johnsson and Varshavsky, 1994). Ubiquitin is a 76 amino acid protein that... 

Remy, I., and Michnick, S. W. (1999). Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays. Proc. Natl. Acad. Sci. USA 96, 5394-5399. 

DNA polymerase enzymes, 22 509-510 DNA polymerases, 22 513 20 447 DNA population, complexity of, 22 503 DNA-protein interactions, 2 7 608 DNA replication, 22 500 DNA restriction fragments, location of specific sequences to, 22 499 DNA sequence information... 

Nucleic acid structures and sequences primary and secondary structure of DNA fragments, translocation of genes between two chromosomes, detection of nucleic acid hybridization, formation of hairpin structures (see Box 9.4), interaction with drugs, DNA triple helix, DNA-protein interaction, automated DNA sequencing, etc. 

Key words High content analysis, Protein fragment complementation. Protein-protein interactions. 

When the membrane is washed with ether to remove all of the cholesterol, the resultant PMR spectrum shows little change in the intensity of the polymethylene chain signal compared with that of the original membrane spectrum. This appears to rule out lipid-cholesterol interaction in this membrane as having a dominant effect upon the polymethylene chain freedom. In the membrane fragments either lipid chain-chain interactions have increased as a result of the protein interaction... 

Michnick, S. W. (2001). Exploring protein interactions by interaction-induced folding of proteins from complementary peptide fragments. Curr. Opin. Struct. Biol. 11, 472-477. 

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