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Random genome fragments

The genome of the Helicobacterpylori bacterium is 1.6 million base pairs in size and encodes 1590 ORFs (Tomb et al., 1997). The comprehensive two-hybrid library screen performed with these ORFs differs from the yeast experiments described above in that the Gal4 activation domain library used consisted of over ten million random genomic fragments (Rain et al., 2001). Thus, the potential problem of full-size ORFs masking protein-protein interactions is reduced. A total of 261 ORFs were fused to the Gal4 DNA binding domain to create a set of baits. These ORFs... [Pg.58]

Random genomic fragments cDNA clones Zea mays (maize) 48... [Pg.164]

Random genomic fragments cDNA clones Lactuca saliva (lettuce) 49... [Pg.164]

UL37 Cloning of small random genome fragments Inhibition of apoptosis Goldmacher et al. 1999... [Pg.3]

Run 10 pL sonicated DNA (from 100 pL total) in 2% agarose gel to verify that random genomic DNA fragments range from 300 to lOOObp. If indicated, repeat the sonication. [Pg.208]

Genomic library A collection of clones made from a set of randomly generated overlapping DNA fragments representing the entire genome of an organism. [Pg.534]

See other pages where Random genome fragments is mentioned: [Pg.88]    [Pg.3]    [Pg.7]    [Pg.88]    [Pg.3]    [Pg.7]    [Pg.118]    [Pg.375]    [Pg.207]    [Pg.114]    [Pg.687]    [Pg.48]    [Pg.195]    [Pg.266]    [Pg.142]    [Pg.96]    [Pg.26]    [Pg.149]    [Pg.385]    [Pg.124]    [Pg.73]    [Pg.60]    [Pg.73]    [Pg.5]    [Pg.8]    [Pg.11]    [Pg.590]    [Pg.117]    [Pg.1235]    [Pg.635]    [Pg.266]    [Pg.63]    [Pg.182]    [Pg.68]    [Pg.13]    [Pg.83]    [Pg.53]    [Pg.357]    [Pg.411]    [Pg.100]    [Pg.70]    [Pg.61]    [Pg.204]    [Pg.256]    [Pg.143]    [Pg.317]   
See also in sourсe #XX -- [ Pg.7 ]



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