Fourier transform vibrational modes, hydrogen-bonded

The IR and Raman spectra of partially hydrated proteins are a rich source of fundamental information on both water and protein species, owing to the sensitivity of vibrational modes to hydrogen bonding. The similar chemistry of water—water and water—peptide interactions requires that there be great accuracy in spectroscopic measurements of the hydration process. Since the review of the field by Kuntz and Kauz-mann (1974), the Fourier transform technique for IR and the tunable laser for Raman spectroscopy have offered important improvements in methodology. 

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