Fourier-transform mass spectrometry MALDI

MALDI = matrix assisted laser desorption, ftms = Fourier transform mass spectrometry TOF = time of flight. 

Stump, M. J. Jones, J. J. Fleming, R. C. Lay, J. O., Jr. Wilkins, C. L. Use of double-depleted 13C and 15N culture media for analysis of whole cell bacteria by MALDI time-of-flight and Fourier transform mass spectrometry. J. Am. Soc. Mass Spectrom. 2003,14,1306-1314. 

Li, Y. Tang, K. Little, D. P. Koster, H. Hunter, R. L. Mclver, R. T. High-resolution MALDI Fourier transform mass spectrometry of oligonucleotides. Anal. Chem. 1996, 68, 2090-2096. 

MALDI mass spectrum of the peptide mellttin from R B. O Connor and C. E. Costello, Application of Multishot Acquisition in Fourier Transform Mass Spectrometry" Anal. Chem. 2000, 72,5125. 

Technological advances of ion-trap mass spectrometers are the ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) and the recently released technique, the Orbitrap Fourier transform mass spectrometry (Hu et al., 2005), which enable the determination of molecular formulae with a high mass resolution and mass accuracy in mixtures. Today these ion-trap mass spectrometers are most frequently coupled with atmospheric pressure ionization (API) techniques such as electrospray ionization (ESI) (e.g., Fievre et al., 1997 Qian et al., 2001 Kujawinski et al., 2002 Llewelyn et al., 2002 Stenson et al., 2002,2003 Fard et al., 2003) or matrix-assisted laser desorption/ionization (MALDI) (e.g., Solouki et al.,... 

Trypsin digests of both wild type HRV virus and the mutant were analyzed using MALDI-TOF and MALDI Fourier transform mass spectrometry (FTMS). For HRV, the mass spectra for both wild-type and mutant were identical except for one peptide occurring at mlz 4700. This corresponds to residues 187-227 in the wild type sequence. The corresponding peak in the mutant mass spectrum occurs at 4783.5 (Fig. 4, inset). This mass difference of 83 Da corresponds exactly to a mutation of a Cys to Trp residue and there are no other possible mutations that would be separated by 83 Da. Since there is only one Cys in the peptide 187-227 at position 199, the mutant can be localized as HRV14-Cysl99Trp, which contains a Trp at position 199 instead of Cys in the wild type. 

Once the polymer molecules have been transferred to the gas phase as ions, they are separated on the basis of their mass-to-charge ratio. Mass spectrometers nsed for MALDI analysis may differ, but for those that are commercially available, separation is effected by TOE Other methods such as quadrupole filter and Fourier transform mass spectrometry (FTMS) may also be used. 

Further performance improvements in analysing nucleic acids could be achieved by the introduction of 3-hydroxypicolinic add as matrix [8] and the introduction of delayed extraction in a linear time-of-flight mass spectrometer [9]. If, for MALDI Fourier transform mass spectrometry, the molecular weight range in analysing nucleic add fragments could be extended further this type of MALDI MS would become of significant value due to the extraordinary resolution possible [10, 11]. In order to reach the sensitivity level necessary for MALDI-TOF MS analysis an amplification step has to be incorporated into the sample preparation process for... 

Figure 2.10 In-cell MALDI Fourier transform mass spectrometry (FTMS).

Atmospheric pressure MALDI-Fourier transform mass spectrometry. 

Borgmann, S. and Wilkins, C.L (2010) Polymer analysis with Fourier transform mass spectrometry, in MALDI Mass Spectrometry (ed. L. Li), Wiley, New York, pp53-85. 

Kellersberger, K.A., Tan, P.V., Laiko, VV, Doroshenko, V.M., Fabris, D. (2004) Atmospheric pressure MALDI-Fourier transform mass spectrometry. Anal. Chem., 76, 3930. 

Fourier transform mass spectrometry (ftms) is an ion cyclotron resonance method (3,4). This method is currently limited to masses less than 20,000. Thus, it works well for lower molecular mass polymers. Often it is used with MALDI. However, the MALDI process is so energetic that the initial velocity of the analytes can cause some intensity difficulties that make the method difficult to quantify. Because of the expense of the magnet required to obtain quahty data with this method, only a few laboratories use this method. 

MALDI-Fourier transform ion cyclotron resonance mass spectrometry MALDI-Q-ion mobility-TOFMS MALDI-Quadrupole-ion-trap-TOF mass spectrometry MALDI-TOF-imaging mass spectrometry MALDI Time-of-flight mass spectrometry Magnetic resonance imaging Matrix solution fixation Moxifloxacin... 

The MALDI-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (MALDI-FT-ICR-MS) and MALDI-Quadrupole-Ion-trap-TOF Mass Spectrometry (MALDI-QIT-TOF-MS) can be used on IMS. The FT-ICR-MS could provide the high resolution, expansive mass range and high sensitivity imaging MS data and good for determining the elemental composition of small molecules [60],... 

In environinontal analysi, there h,-is been incre.-ts-ing use of TOF. quadrupolo. ion-trap, and Fourier transform mass spectrometers in addition to desorption ioni/ation methods such as MALDI. - Detection and quantitative determination of such widely diverse contaminants as porfluoroorganics. polybromi-nated diphenyl ethers, pharmaceuticals, byproducts of water disinfection, pesticides, algal toxins, surfactants, methyl-(-butyl ether, arsenic, and various microorganisms are now carried out using mass spectromelrle methods. Mass spectrometry is also u.sed to determine compounds of interest in homeland security."... 

In the last decade there has been a fast progress in the field of mass spectrometry employing soft ionization techniques such as electrospray ionization mass spectrometry (ESI-MS), Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS), and matrix-assisted laser desorption ionization mass spectrometry (MALDI)-MS. These tools provide molecular level information on humic substances. 

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