Fluorescent Visualization

Han P, Zhou X, Huang B et al (2008) On-gel fluorescent visualization and the site identification of S-nitrosylated proteins. Anal Biochem 377 150-155... 

Beatty KE, Liu JC, Xie F et al (2006) Fluorescence visualization of newly synthesized proteins in mammalian cells. Angew Chem Int Ed 45 7364-7367... 

Easy fluorescent visualization of resin with binding disulfide... 

Fluorescent Visualization of Synaptic Vesicle Fusion and Recycling. 31... 

FIGURE 3.12 Simulation (left column) and photobleached-fluorescence visualization (right column) of an analyte band traveling around a constant-radius corner. In both cases the channel is 250 pm wide. In the experiments, the channels were - 40 pm deep [535]. Reprinted with permission from the American Chemical Society. 

Fluorescent Visualization of Macromolecules in Drosophila Whole Mounts... 

V. Wittmann, A. K. Datta, K. M. Koeller, and C.-H. Wong, Chemoenzymatic synthesis and fluorescent visualization of cell-surface selectin bound sialyl Lewis X derivatives, Chem. Eur. J., 6 (2000) 162-171. 

ALA also appears to have potential for clinical use as a photodiagnostic or photodetection agent. Fluorescence visualized after intravesical instillation of ALA shows some specificity for mucosal lesions including cancer and dysplasias, although false positives caused by inflammatory areas are also seen. However, it is the improvement in sensitivity rather than in specificity that suggests that ALA photodiagnosis could be a useful tool for guiding biopsies. 

Fluorescence microscopy Fluorescence visualization Fluorescent microscopy Fluorescent visualization... 

Figure 8.6 Vapor phase fluorescence visualization chamber. (Photograph courtesy of Analtech.)...

This remains a standard technique. TLC plates are impregnated with UV fluorescence material at 254 nm (typically zinc silicate). Upon exposure, the nucleic acids absorb at 254 nm, and therefore, quench, so they appear black against a blue-green background of fluorescence. Visual error rates for quantification by UV remain high (30%), Some scanning detectors employ U V which can be applied to TLC plates, and provide better quantitative data. 

Laser-induced fluorescence Visualization by tracer material that emits fluorescent radiation upon excitation by laser light. 

Rg. 3 Visualization of the rat brain (neocortex) microglia using confocal laser miaoscopy (orthogonal projection of 15 pm z-stack). Confocal laser microscope LSM 710 (Zeiss, Oberkochen, Germany). Ibal -immunocytochemistry, fluorescence visualization using Cy3... 

Fig. 4 3D reconstruction of the rat neocortical microgliocyte (cell body and processes) created from 15 pm z-stack using the confocal laser microscope LSM 710 (Zeiss) and the software ZEN 2012 Gray (Zeiss). Ibal immunocytochemistry, fluorescence visualization—Cy3, nuclear counterstaining—SYTOX Green... 

The following chiral reagents were employed for diastereomer formation before sample application and chromatography on silica gel or silica gel G TLC plates (L)-leucine Af-carboxyanhydride for D,L-dopa-carboxyl- " C separated with ethyl acetate/formic acid/water (60 5 35) mobile phase and detected by ninhydrin [7 f 0.38 (d)/0.56 (l)] [43] Af-trifluoroacetyl-L-prolyl chloride for D,L-amphetamine separated with chloroform/methanol (197 3) and detected by sulfuric acid/formaldehyde (10 1) (Rf 0.49 (d)/0.55 (l)) [44] Af-benzyloxycarbonyl-L-prolyl chloride for D,L-methamphetamine separated with n-hexane/ethyl acetate/acetonitrile/diisopropyl ether (2 2 2 1) and detected by sulfuric acid/formaldehyde (10 1) [/ f 0.57 (l)/0.61 (d)] [44] (l/ ,2/ )-(-)-l-(4-nitrophenyl)-2-amino-1,3-propanediol (levobase) and its enantiomer dextrobase for chiral carboxylic acids separated with chloroform/ethanol/acetic acid (9 1 0.5) and detected under UV (254 nm) light R[ values 0.63 and 0.53 for 5- and / -naproxen, respectively) [45] (5)-(4-)-a-methoxyphenylacetic acid for R,S-ethyl-4-(dimethylamino)-3-hydroxybutanoate (carnitine precursor) with diethyl ether mobile phase [/ f 0.55 R)/0J9 (5)] [46] and (5)-(4-)-benoxaprofen chloride with toluene/acetone (100 10, ammonia atmosphere) mobile phase and fluorescence visualization (Zeiss KM 3 densitometer 313 nm excitation, 365 nm emission) (respective R values of R- and 5-isomers of metoprolol, oxprenolol, and propranolol were 0.24/0.28, 0.32/0.38, and 0.32/0.39) [47]. 

---