Fluorescent transfer, using

Filipescu and co-workers(84-88) have also examined intramolecular singlet and triplet energy transfer using fluorescence and phosphorescence as a gauge for energy transfer. Lamola<87) has repeated one of the studies... 

HPLC with microchip electrophoresis. Capillary RPLC was used as the first dimension, and chip CE as the second dimension to perform fast sample transfers and separations. A valve-free gating interface was devised simply by inserting the outlet end of LC column into the cross-channel on a specially designed chip. Laser-induced fluorescence was used for detecting the FITC-labeled peptides of a BSA digest. The capillary HPLC effluents were continuously delivered every 20 s to the chip for CE separation. 

Using picosecond flash spectroscopy Gupta et al. 2k) reported for 2-hydroxyphenylbenzotriazole in ethanol a short-lived transient (6 ps) followed by a transient absorption whose lifetime is estimated to be 600 ps. The authors assigned the short-lived transient to the "vertical singlet" while the long-lived transient is presumably the "proton transferred species". These measurements of transient absorptions with the picosecond flash method confirm our results derived from the fluorescence emission using the phase fluorimetric method. 

Stoner-Ma D, laye AA, Matousek P, Towrie M, Meech SR, Tonge PJ (2005) Observation of excited-state proton transfer in green fluorescent protein using ultrafast vibrational spectroscopy. I Am Chem Soc 127 2864—2865... 

Xia, Z. and Liu, Y. (2001). Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes. Biophys. J. 81, 2395-402. 

Szollosi, J., Matyus, L., Tron, L Balazs, M Ember, I., Fulwyler, M. J., and Damjanovich, S. (1987) Flow cytometric measurements of fluorescence energy transfer using single laser excitation. Cytometry 8, 120-128. 

Methods for the Detection of Antigens/Antibodies Equilibrium and kinetic inhibition assays based upon fluorescence polarization, 70, 3 fluorescence excitation transfer immunoassay (FETI), 70, 28 indirect quenching fluoroimmunoassay, 70, 60 the homogeneous substrate-labeled fluorescent immunoassay, 70, 79 fluorescence immunoassays using plane surface solid phases (FIAPS), 70, 87. 

Enzyme working solution (10 pL, as determined above or as specified by the reagents and solutions ) is pipeted into wells Al-Hl and A2-H2 of a flat-bottomed, 96-well fluorescence plate. Using 8 tips on a 12-channel pipeter, 190 pL of substrate solutions in the polypropylene plate are transferred simultaneously to the corresponding well of the fluorescence plate with enzyme. 

However, the complete transfer of the correlations does not necessary mean that the two-photon correlations are stored in the pure TPE state. This happens only for the small sample model and two atoms with significantly different transition frequencies, where the steady state is the pure TPE state. For identical atoms separated by a finite distance r 2, a part of the correlations is stored in the antisymmetric state. This fact can lead to an interesting modification of the interference pattern of the fluorescence field. Using the steady-state solutions (114), we find that the visibility in the interference pattern is given by [58]... 

Fluorescence imaging is the most powerful technique currently available for continuous observation of the dynamic intracellular processes of living cells. Fluorescein is widely employed as the core of various fluorescence probes used in imaging important biological effectors. Despite the extensive use of fluorescein derivatives and the importance of the applications, the mechanism that controls the quantum yield of fluorescence has not been fully established. I report herein photoinduced electron transfer (PeT) mechanism that can control the fluorescence quantum yields of fluorescein and boron dipyrromethene (BODIPY) derivatives. 

Proton transfer processes are specially important excited state properties, and several detailed time resolved studies have been reported. Time resolved fluorescence studies of excited l-naphthol-3,6-disulphonate shows there is geminate recombination by proton transfer. Another detailed study is the examination of proton transfer and solvent polarization dynamics in 3-hydroxyflavone . The dynamics of proton transfer using a geminate dissociation and recombination model has also been investigated with 8-hydroxypyrene-l,3,6-trisulphonate 5 and also with... 

Fig. 85 Fluorescence spectra from the target polymer surface of (a) poly(ethyl methacrylate) PEMA and (b) polyfbutyl methacrylate) PBMA, following 355-nm laser-induced pyrene transfer using ablation of a triazene polymer. Irradiation dose 10 pulses, 100 mj cm-2. REPRINTED WITH PERMISSION OF [Ref. 360], COPYRIGHT (1998) Elsevier Science...

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