Fluorescent imaging fluorescence resonance energy transfer

Isik N, Hereld D, Jin T (2008) Fluorescence resonance energy transfer imaging reveals that chemokine-binding modulates heterodimers of CXCR4 and CCR5 receptors. PLoS ONE... 

Clegg RM (1996) Fluorescence resonance energy transfer. In Wang XF, Flerman B (eds) Fluorescence imaging spectroscopy and microscopy. John Wiley, New York, pp 179-252... 

Clegg, R. M. (1996). Fluorescence resonance energy transfer. In Fluorescence Imaging. Spectroscopy and Microscopy. Vol. 137. (Wang, X. F. and Herman, B., eds.). John Wiley Sons, New York, pp. 179-252. 

Jovin, T. and Arndt-Jovin, D. (1989). FRET microscopy Digital imaging of fluorescence resonance energy transfer. Application in cell biology. In Cell Structure and Function by Microspectrofluorometry (Kohen, E., JG, H. and Ploem, J., eds.). Academic Press, London, pp. 99-117. 

The term filterFRET here refers to intensity-based methods for calculating fluorescence resonance energy transfer (FRET) from sets of images of the preparation collected at different excitation and/or emission wavelength. The term is not intended to imply that interference filters are actually present in the setup very similar considerations apply when donor- and acceptor fluorophores are spectrally resolved by other means, such as monochromators or spectral detectors. 

Ecker, R. C., de Martin, R., Steiner, G. E. and Schmid, J. A. (2004). Application of spectral imaging microscopy in cytomics and fluorescence resonance energy transfer (FRET) analysis. Cytometry A 59, 172-81. 

Kenworthy, A. K. (2001). Imaging protein-protein interactions using fluorescence resonance energy transfer microscopy. Methods 24, 289-96. 

The upgrade of a frequency-domain fluorescence lifetime imaging microscope (FLIM) to a prismless objective-based total internal reflection-FLIM (TIR-FLIM) system is described. By off-axis coupling of the intensity-modulated laser from a fiber and using a high numerical aperture oil objective, TIR-FLIM can be readily achieved. The usefulness of the technique is demonstrated by a fluorescence resonance energy transfer study of Annexin A4 relocation and two-dimensional crystal formation near the plasma membrane of cultured mammalian cells. Possible future applications and comparison to other techniques are discussed. 

Elangovan, M., Day, R. N. and Periasamy, A. (2002). Nanosecond fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy to localize the protein interactions in a single living cell. J. Microsc. 205, 3-14. 

Cohen-Kashi, M., Namer, Y. and Deutsch, M. (2006). Fluorescence resonance energy transfer imaging via fluorescence polarization measurement. J. Biomed. Opt. 11, 34015. 

Sekar RB, Periasamy A (2003) Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations. J Cell Biol 160 629-33... 

Sagot, I., Bonneu, M., Balguerie, A. and Aigle, M. (1999). Imaging fluorescence resonance energy transfer between two green fluorescent proteins in living yeast. FEES Letters, 447, 53-57. 

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