Fluorescence spectra deconvolution

The frequency (o and gate pulse time-delay td dependent fluorescence up-conversion signal F(cojd) have been theoretically modeled by using an overdamped multi-mode Brownian oscillator model [5], The deconvoluted fluorescence spectrum is given by... 

Unfortunately, no lead sensors have been developed to date that meet all of these criteria. The system that has been used most extensively to quantitate lead levels in vivo is the fluorescent sensor Indo-1 (Fig. 24) (443 45). Although originally developed as a calcium dye (446) log iCfc[Ca(II)] = 6.6, Indo-1 (2-[4-[bis(carboxymethyl)amino]-3-[2-[2-[bis(carboxymethyl)amino]-5-methyl-phenoxy]ethoxy]phenyl]-lH-indole-6-carboxylic acid) binds lead quite tightly (log ffo[Pb(II)] = 10.5 and exhibits a very different fluorescence emission spectrum when bound to lead than when free in solution or bound to calcium (Fig. 24). As a result, Indo-1 can be used to determine whether lead is present in cells, even in the presence of excess calcium, provided that the fluorescence spectrum is deconvoluted to account for calcium interference and all of the possible equilibria are taken into consideration (443, 445). The main drawback of the Indo-1 detection system is that the dye is almost completely quenched when bound to lead (Fig. 24, spectrum b), making it difficult to quantitate the amount of free lead present. 

Figure 6.14 (a) The parallel optical analysis of antigens in a well array using the fluorescence of different-sized quantum dots (B) Fluorescence spectrum observed for the four analytes (concentration 1 pgmr ) by the different-sized QDs (spectrum a), and deconvoluted spectra... 

Figure 12.9 Spectra deconvolution by using the subtraction technique to elicit the response of membrane bioreactor when exposed to different concentrations of pollutant (3-chloro-4-methylaniline). Fluorescence spectra acquired (a) in the presence of 500mg/L of pollutant, (b) in the presence of 250mg/L of pollutant and (c) subtraction fluorescence spectrum.

Deconvolution of the initial spectrum, when PC fluorescence is at a maximum, resolved four PC chro-mophore components with emission bands at 623,639,644 and 652 nm [see Fig. 12 (B)]. In the 665 and 685 nm regions the red shift of the maxima with time could be accounted for by two component bands... 

Fig. 12. (A) Time-resolved fluorescence spectra of A. nidulans phycobilisomes measured at 77 K. Excitation by 6-ps, 580-nm argon laser pulse. Three small ticks in the topmost spectrum (at 932 ps) indicate locations of maximum fluorescence at 0 ps. (B) Rise and decay of various fluorescent components derived from deconvolution of the fluorescence spectra. Assignment of individual fluorescent components are shown in the right margin. (C) Energy flow among individual chromophores in the phycobilisomes. The asterisk in (B) and (C) indicates a linker polypeptide is attached to the trimer. See text for discussion. Figure source Mimuro (1989) Studies on excitation energy How in the photosynthetic pigment system structure and energy transfer mechanisms. Bot Mag Tokyo 103 244.

Fig. 11 Laser-induced fluorescence spectra recorded from Napl-doped PMMA samples (1.2 wt%) after their irradiation with a single pump pulse at 248 nm at laser fluences below a, and above b the corresponding ablation thresholds. For comparison purposes, the spectra have been scaled. The figure also illustrates the approximate deconvolution of the probe spectrum into the emission bands of the suggested species (the Nap2 spectrum is recorded in the photolysis of high-concentration NapI solution, while the NapH/PMMA spectrum is recorded from PMMA doped with 0.08 wt% NapH)...

McPherson, A., Friedman, M. L. and Halsall, B. 1984, Crystallization of ai-acid lipoprotein. Biochemical Biophysical Research Communications 124, 619-624. Meagher, J. L., Beechem, J. M., Olson, S. T. and and Gettins, P. G. W. 1998, Deconvolution of the fluorescence emission spectrum of human antithrombin and identification of the tryptophan residues that are responsive to heparin binding. Journal of Biological Chemistry 273, 23283-23289. 

Fig. 2 Rise and decay patterns of the fluorescence components resolved by deconvolution of the spectra as shown in Fig. ID. Each point was calculated by the relative height in the spectrum and actual counts of photons. Broken line the excitation pulse profile. The bar over each curve indicates the maximum point of intensity.

Fig. 2. Time-resolved fluorescence spectra of P. tamarensis at —196°C (A), their deconvoluted patterns (B) and rise and decay curves of individual fluorescence components (C). In (A), each time-resolved spectrum was shown after normalization to the maximum intensity. In (C), vertical line shows the time zero and broken lines, pulse profile. Small bars over the decay curves indicate the time when the maximum intensity was observed.

Fig. 26 UV-vis and fluorescence spectra of pseudogeminal and pseudopara phenylene vinylenes and model unstacked oligomer (a) stUbene (PV2) analogs (b) distyrylbenzene (PV,) analogs (inset long wavelength peak from deconvolution of the emission spectrum ofpg -CPlPVjt) [125]...

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