Fluorescence quench thin-layer scanning

Figure 5. Thin-layer chromatogram of tris (2,3-dibromopropyl)phosphate on fluorescein impregnated silica gel solvent methylene chloride. Scan in fluorescence quench mom. (a) 3.7 /ig TRIS (b) 0.44 ttg TRIS.

The Zeiss PMQ 3 chromatogram analyzer is probably the most versatile thin-film scanner available (Fig.3.13). The system can be used for reflectance, transmission, simultaneous reflectance and transmission and fluorescence quenching. It has two direct fluorescence modes, one with filter emission and surface illumination at a direction of 90° to the surface of the plate, and the other with 45° illumination and monochromatic emission. The instrument can be used for scanning thin-layer chromatograms, paper... 

Densitometry is the instrumental measurement of visible or UV absorbance, fluorescence, or fluorescence quenching directly on the thin layer. Measurements are made either through the plate (transmission), by reflection from the plate, or by reflection and transmission simultaneously, and either single-beam, doublebeam, or single-beam-dual wavelength operation of scanning instruments has been used. 

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