Rapid scanning fluorescence

Retzik, M. and Froehlich, P., Extending the capability of luminescence spectroscopy with a rapid-scanning fluorescence spectrophotometer, Am. Lab., March, 68, 1992. 

A number of systems for making measurements on living cells have been described. An DMA has been used to obtain subnanosecond fluorescence spectra and anisotropy . This has been used to study the stimulus induced secretion of acriflavine-loaded blood platelet granules. A rapid scanning fluorescence spectrometer has... 

Johnson, L. W. Callis, J. Christian, G. D. Rapid Scanning Fluorescence... 

Fluorescence detection is not very often used in phenohc acid analysis, but, in cases when very low concentrations of some analytes or many interfering compounds are present in extracts, the combination of UV and DAD with fluorescence detector could be valuable. However, the same problems could arise as in the UV detection, i.e., establishing the correct excitation and emission wavelengths, as large differences for several phenolic acids were observed. In this case, the rapid scanning fluorescence detector, in combination with DAD, is available for applying multiple excitation and emission wavelengths. ... 

White et al. have reported a rapid fluorimetric determination of chlorpromazine by an in situ photochemical oxidation [139]. Variable-angle synchronous scanning fluorescence spectroscopy has also been applied to the determination of chlorpromazine and its sulfoxide [140]. 

The atmospheric chemistry of trifluoroacetyl radical CF3CO is not well known. It was implicated to explain the products observed98 in photolysis of hexafluoroacetone in the presence of Br2 and Cl2, but its first direct observation came from rapid-scan infrared spectroscopic studies in a matrix99, and more recently its laser-induced fluorescence spectrum has been observed100 the band origin for the first excited state of the radical appears at 384 nm. A weak UV absorption band which onsets at 250 nm and continues to increase in intensity below 200 nm has been attributed to CF3C0101. 

Figure 5.14. Schematic diagram of a fluorescence detector with rapid scanning monochromators for programmable selection of excitation and emission wavelengths.

Fig. 2. Uniform stimulus and switching time, (a) A solution of 10 pM DMNB-caged fluorescein is transported by the flow from /effto right. Photochemical release of the dye is initiated inside the white rectangle by rapid scanning of a 405-nm laser, (b) The fluorescence intensity is measured in a small rectangular region around the position of the cell in (a). The average intensity is shown in the course of time. Less than 1 s is required for switching of the dye concentration at the location of the cell. Reproduced with permission from ref.f Copyright 2007 American Chemical Society.

Kauppinen T, Khajehzadeh N, Haavisto O (2014) Laser-induced fluorescence images and Raman spectroscopy studies on rapid scanning of rock drill core samples. Int J Miner Proc 132 26-33 Lamprecht G, Himan H, Snyman L (2007) Detection of diamond in ore using pulsed laser Raman spectroscopy. J Miner Proc 84 262-273... 

Both instrument design and capabilities of fluorescence spectroscopy have greatly advanced over the last several decades. Advancements include solid-state excitation sources, integration of fiber optic technology, highly sensitive multichannel detectors, rapid-scan monochromators, sensitive spectral correction techniques, and improved data manipulation software (Christian et al., 1981 Lochmuller and Saavedra, 1986 Cabaniss and Shuman, 1987 Lakowicz, 2006 Hudson et al., 2(X)7). The cumulative effect of these improvements have pushed the limits and expanded the application of fluorescence techniques to numerous scientific research fields. One of the more powerful advancements is the ability to obtain in situ fluorescence measurements of natural waters (Moore, 1994). 

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