Fluorescence probe method

DD can be monitored by a variety of experimental techniques. They involve thermodynamic, dilatometric, and spectroscopic procedures. Molecular dynamics (MD) simulations also become applicable to self-assembled systems to some extent see the review in Ref. 2. Spectroscopic methods provide us with molecular parameters, as compared with thermodynamic ones on the macroscopic level. The fluorescence probing method is very sensitive (pM to nM M = moldm ) and informs us of the molecular environment around the probes. However, fluorescent molecules are a kind of drug and the membrane... 

Vasilescu, M., Angelescu, D., Almgren, M., Valstar, A. (1999). Interactions of globular proteins with surfactants studied with fluorescence probe methods. Langmuir, 15, 2635-2643. 

Kato, A. and Nakai, S. 1980. Hydrophobicity determined by a fluorescence probe method and its correlation with surface properties of proteins. Biochim. Biophys. Acta 624, 13-20. 

Most fluorescence probe methods can accommodate more than ten samples per day. However, other methods such as detergent binding, hydrophobic interaction chromatography, and hydrophobic partitioning can accommodate only one or two protein samples a day. 

Fluorescence Of Monolayers Containing Pyrene-Labeled Probes. A fluorescence probe method was also used as a complementary technique to study the thermodynamics of SA film formation. Mixed monolayers containing the fluorescence probe pyrene hexadecanoic acid, Py-C16, in host fatty acids of different lengths were prepared by adsoiption from solutions containing mostly the host fatty acid and a small fraction of Py-C16 (approximately 1 to 5 mol %). All monolayers were prepared under equilibrium adsoiption conditions. For fluorescence measurements only A1 substrate was used because when glass is used an impurity fluorescence from glass interferes with the pyrene fluorescence. 

In this chapter, we concentrate on luminescence approaches that are most suitable for stilbenes to be molecular probes and are based on specific and nonspecific labeling, competition, solvatochromism, experimental molecular dynamics, and singlet-singlet and triplet-triplet energy transfer. A general survey is made of the physical principles and application of the fluorescence probe methods stressing on latest developments in this area. 

Both dye solubilization and fluorescence probe methods have been used to study protein/ surfactant interaction. For example, data of Steinhardt et al. (137), who studied the solubilization of dimethylaminoazobenzene in ovalbumin-SDS mixtures, are shown in Figure 33. Solubilization occurs at concentrations of SDS well below the c.m.c., indicating that in the presence of ovalbumin the formation of nonpolar clusters is highly favorable. Unlike... 

The fluorescent probe methods were used to determine miCToviscosity (T ,) of potassium A-acylalaninates and potassium A-acylvalinates micelles. The results obtained show that 11 on the micellar surface is larger in A-acylalaninates than in 7V-acylvalinates, whereas in the micellar core remains same in the micelles of the two surfactant series. The values of 11 of SDS, lithium dodecyl sulfate (LDS), CTACl, and CjjEg, determined at 15°C by monomer/excimer intensity ratio and excimer lifetime of dipyrenylpropane dissolved in micelles, are 19, 19, 39, and 57 cP, respectively. The fluorescent probe technique was used to determine r m values of SDS, CTABr, and CTACl micelles at different applied pressure. The derived values of SDS, CTABr, and CTACl micelles at 25°C and atmospheric pressure are 12,47, and 27 cP, respectively. The addition... 

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