Laser induced fluorescence polarisation

There have been several new developments in fluorescence polarization immunoassay (FPIA). Lackowicz and Terpetschnig reviewed the use of long-lifetime metal-ligand complexes in fluorescence polarization assays [154, 155]. New complexes with Re(l) and Ru(II) were described for the highly sensitive detection of high-molecular weight analytes by FPIA. Laser-induced fluorescence polarisation detection has been used by Yatscoff and coworkers in capillary electrophoresis detection (CE-LIFP) [156]. For the analyte cyclosporin picomolar detection limits were attained. 

The author became interested in collision-induced state-to-state processes as a result of experiments in which narrow-line, circularly polarised laser radiation was used to excite fluorescence from diatomic molecules in collision cells. The laser excites known multipolar moments of nij distribution among specific j states of an excited vibronic state. Wavelength and polarisation analysis of the emission allowed the population and orientation multipoles of nij among states accessed by elastic and inelastic collisions to be determined from measured intensity and degree of circular polarisation. An example is shown in Fig. 1, from which it is evident that the initial asymmetric nij distribution is maintained through elastic and inelastic collisions [13] even when substantial amounts of vibrational and rotational energy... 

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