Fluorescence line-narrowing

The development of tunable, narrow-bandwidtli dye laser sources in tire early 1970s gave spectroscopists a new tool for selectively exciting small subsets of molecules witliin inhomogeneously broadened ensembles in tire solid state. The teclmique of fluorescence line-narrowing [1, 2 and 3] takes advantage of tire fact tliat relatively rigid chromophoric... 

Larsen OFA, IS Kozin, AM Rija, GJ Stroomberg, JA de Knecht, NH Velthorst, C Gooijer (1998) Direct identification of pyrene metabolites in organs of the isopod Porcello scaber by fluorescence line narrowing spectroscopy. A a/ Chem 70 1182-1185. 

FLNS Fluorescence line-narrowing GPC Gel permeation chromatography (see... 

Site-selection spectroscopy Maximum selectivity in frozen solutions or vapor-deposited matrices is achieved by using exciting light whose bandwidth (0.01-0.1 cm-1) is less than that of the inhomogeneously broadened absorption band. Lasers are optimal in this respect. The spectral bandwidths can then be minimized by selective excitation only of those fluorophores that are located in very similar matrix sites. The temperature should be very low (5 K or less). The techniques based on this principle are called in the literature site-selection spectroscopy, fluorescence line narrowing or energy-selection spectroscopy. The solvent (3-methylpentane, ethanol-methanol mixtures, EPA (mixture of ethanol, isopentane and diethyl ether)) should form a clear glass in order to avoid distortion of the spectrum by scatter from cracks. 

Lochhead and Bray (1995) studied Eu3+ doped sodium disilicate glass with a high-pressure fluorescence line-narrowing technique. This technique was used to characterize the local structure of the Eu3+ ions up to a pressure of 21 GPa. For the crystal-field analysis they assumed a C2v site symmetry which allowed for a complete splitting of the crystal-field components. The crystal-field strength was determined according to eq. (11). The effect of pressure... 

T.M.H. Creemers, C. de Caro, R.W. Visschers, R. van Grondelle, S. Volker, Spectral hole burning and fluorescence line narrowing in subunits of the light harvesting complex LHl of purple bacteria. J. Phys. Chem. B 103, 9770-9776 (1999)... 

The time-resolved spectroscopy of Cr doped spinel and petalite-like phase types glass-ceramics enables a more accurate distinction of the and T2 energy levels of Cr(III) and of the equilibrium between the population of two levels, see Fig. 5, which presents the time-resolved spectra of spinel type glass-ceramics excited at 680 nm at room temperature Glass-ceramics derived from boro-silicate glasses containing Cr(III) were also recently studied by laser spectroscopy and fluorescence-line narrowing. 

The rate constant (36) and (37) as a function of temperature correlated well with the experimental data obtained for the carboxylic acid protons of crystalline perprotobenzoic acid and ring-deuterobenzoic acids by nuclear magnetic resonance 7) [75] and inelastic neutron scattering (for an analysis of the experiment see Refs. 76 and 77). It should be noted that some of the major parameters of the model (for instance,. /) allowed the direct determination by fluorescence line narrowing technique. 

Both this fluorescence line narrowing and hole burning spectroscopy are conceptually trivial. Still, they can provide very useful information on the homogeneously broadened lines as illustrated in Fig. 18.11. What makes life less simple and more interesting is that other dynamical effects can express themselves in this... 

The structure of the adduct was established by fluorescence line narrowing spectrometry (FLNS). Recovery and precision data were not reported (Rogan et al. 1990). 

The compressibility of a protein may also be obtained from fluorescence line-narrowing spectroscopy at 10 K low temperatures. Under these conditions one does expect the hydrational changes not to play a very prominent role. Nevertheless the compressibilities that are obtained under such conditions are of the same order of magnitude as those obtained at ambient conditions [34]. This points to important contributions from the cavities to the compressibility and the thermal expansion. The observed pressure-induced amorphization in inorganic substances [35], liquid crystals [36], synthetic polymers [37,38] and starch [9] also support this hypothesis. 

Fig. 16 Steady state fluorescence line narrowing in neat [Rh(bpy)3][NaCr(ox)3]C104 as a function of temperature between 1.8 and 4.2 K (adapted from [25])...

Seber et al. (56) and Flach et al. (57) applying the time resolved fluorescence line narrowing have observed directly the migration of energy within the 3P0 state of Pr3+ LaF3. 

Kushida, T., Takushi, E. Determination of Homogeneous Spectral Widths by Fluorescence Line Narrowing in Ca(P03)2 Eu3+, to be published in Phys. Rev. 1976. 

Orrit M, Bernard J and Personov R I 1993 High-resolution spectroscopy of organic molecules in solids from fluorescence line narrowing and hole burning to single molecule spectroscopy J. Phys. Chem. 97 10 256-68... 

Lee H W H, Walsh C A and Payer M D 1985 Inhomogeneous broadening of electronic transitions of chromophores in crystals and glasses analysis of hole burning and fluorescence line narrowing experiments J. Chem. Phys. 82 3948-58... 

Murdoch KM, Edelstein NM, Boatner LA, Abraham MM (1996) Excited state absorption and fluorescence line narrowing studies of inLuP04. J ChemPhys 105 2539-2546 Muto T, Meyrowitz R. Ponuner AM, Murano T (1959) Ningyoite, a new uranous phosphate mineral from Japan. Am Mineral 44 633-650... 

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