Flowing chromatogram

Figure 2. Comparison of narrow-fraction and reverse-flow chromatogram widths...

Flowing Chromatogram, on Powdered Cellulose, of 1.6 g. of the Sugars from the Incomplete Hydrolysis of the Gum of the Sterculia setigera rree ... 

The powdered-cellulose, flowing chromatogram of an unfermentable residue from the mixture obtained by the action of heat on n-fructose yielded a sirup containing a ketose having a mobility greater than that of D-fructose it may be n-psicose. D-Psicose (identified as the phenylosazone... 

Flowing Chromatogram on Powdered Cellulose of the Sugars from the Hydrolysis of 1.082 g. of Frog-spawn Mucin ... 

As an example of a continuous flow chromatogram Fig. 9.3.6 shows a stack of NMR spectra (right) as a function of elution time for five vitamin A acetate isomers on a cyanopropyl column in n-heptane recorded at a flow rate of 0.2 ml/min [Albl]. The first two peaks somewhat overlap in the ultraviolet (UV) detected chromatogram (left), but in the NMR spectra (right) the peaks are well separated due to different chemical shifts. Forty-eight scans were added for each trace, so that the time resolution was 62 s. 

Figure 4 Quantification of separated Cd species in fish MTs (real sample) by AE-FPLC-ID-ICP-MS. Cd was used as spike. Inset shows the transformation of intensity signals into mass flow chromatogram (by means of ID equation). The quantification of Cd bound to each metalloproteins (numbered from 1 to 5) could be achieved by this technique. (Rodn guez-Cea A, Fernandez de la Campa MR, Blanco Gonzalez E, Andon B, and Sanz-Medel (2003) Metal spedation analysis in eel Anguilla anguilla) metallothioneins by anionic exchange FPLC-isotope dilution-ICP-MS. Journal of Analytical Atomic Spectrometry 18 1357-1364 reproduced from The Royal Society of Chemistry.)...

Fig. 2 A reversed-flow chromatogram showing sample peaks for the diffusion of 1-butene into nitrogen (corrected volume flow rate V = 0.33 cm /sec) at 343.2 K and 1 atm.

Figure 8.20 Sulfur mass flow chromatogram of gas oil reference material BCR CRM 107 obtained by species-unspecific CC-ICP-IDMS. Major peaks identified by EI-MS-MS ...

Figure 8.21 Mass flow chromatograms of molybdenum, zinc, and copper complexes with humic substances obtained by SEC-ICP-IDMS for sewage water from two different sewage plants. Reproduced with permission from [104].

Figure 8.25 Sulfur mass flow chromatogram for a protein standard solution as obtained by species-unspecific micro-HPLC—ICP-IDMS and conversion into the amounts of the separated proteins based on the known number of sulfur atoms per protein molecule. Reproduced with permission from [111],...

Figure 4.9 Sulfur mass flow chromatogram obtained from HSA tryptic digest by the pre-column isotope dilution technique. 2007 American Chemical Society.

Retention Behavior. On a chromatogram the distance on the time axis from the point of sample injection to the peak of an eluted component is called the uncorrected retention time The corresponding retention volume is the product of retention time and flow rate, expressed as volume of mobile phase per unit time ... 

With eluent MeOH H O = 40 60% w (flow rate -1 ml/min) full sepai ation was achieved within acetylated and non-acetylated on amino group of eight thioesters of 4-aminobenzenthiosulfinic acid with retention in next order for R - = -CH3, -C,H3,-CH,-CH=CH, -C3H3by RP-HPLC on Spherisorb-ODS-2 (250x4,6 mm). The chromatograms were obtained at 254 and 289 nm. Retention was generalized by In = In - S-(p (cp - MeOH volume pai t in range near 0.4-0.6) as shown in Fig. 

The column used in the upper chromatogram was 24 cm long, 4.6 mm I.D. the solvent was tetrahydrofuran, the solute benzene and the flow rate 1 ml/min. The column used in the lower chromatogram was 1 m long, 1 mm I.D. using the same solvent and solute but at a mobile phase flow rate of 40 ml/min. It is seen that the reduction in cell volume has a dramatic effect on peak shape. The large 25 pi cell... 

FIGURE 16.1 Preparative SEC of short-chain (scb) branched glucans of small" (<3S /u.m) starch granules of potato species Ostara separated on Sephacryl S-1000 (88 X 2.6 cm) eluent 0.005 M NaOH the normalized chromatogram (area = 1.0) was constructed from an off-line determined carbohydrate content of succeeding 5-ml fractions flow rate 0.67 ml/mln V d = 185 ml, V, = 460 ml fraction I high dp fraction fraction 2 low dp fraction. 

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