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Fraction Fractionation

Fraction Fraction Fraction Fraction Fraction Fraction Fraction Fraction 8 Pellet... [Pg.321]

Fraction Fraction IAS Fraction Fraction Fraction Fraction CLk at peak of (%)... [Pg.203]

FIGURE 16.1 Preparative SEC of short-chain (scb) branched glucans of small" (<3S /u.m) starch granules of potato species Ostara separated on Sephacryl S-1000 (88 X 2.6 cm) eluent 0.005 M NaOH the normalized chromatogram (area = 1.0) was constructed from an off-line determined carbohydrate content of succeeding 5-ml fractions flow rate 0.67 ml/mln V d = 185 ml, V, = 460 ml fraction I high dp fraction fraction 2 low dp fraction. [Pg.467]

Most purification procedures for a particular protein are developed in an empirical manner, the overriding principle being purification of the protein to a homogeneous state with acceptable yield. Table 5.5 presents a summary of a purification scheme for a selected protein. Note that the specific activity of the protein (the enzyme xanthine dehydrogenase) in the immuno-affinity purified fraction (fraction 5) has been increased 152/0.108, or 1407 times the specific activity in the crude extract (fraction 1). Thus, xanthine dehydrogenase in fraction 5 versus fraction 1 is enriched more than 1400-fold by the purification procedure. [Pg.130]

Foam fractionation Fractionation, see Distillation Metal separation... [Pg.351]

Figure 4. Chromatogram on HW-40 S column of the low molecular weight fraction (Fraction 3) from the HW-55 S column chromatography of the EPG-digested alkali extract. Figure 4. Chromatogram on HW-40 S column of the low molecular weight fraction (Fraction 3) from the HW-55 S column chromatography of the EPG-digested alkali extract.
The results obtained after purification of pectinesterase preparation using columns with various gels certified that the active fraction (fraction 1 has greater molecular weight) was not subjected to further separation on the tested gels. Components of fraction 1 increased an activity of elute. That can be explained by their acid properties. Pectinesterase activity was accumulated in fraction 1. Activity of the other components of pectolytic complex was not found in the other studied probes of fraction 1. [Pg.951]


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See also in sourсe #XX -- [ Pg.165 ]




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