Flow cytometry technique

While more commonly used to count or otherwise characterize cells for medical applications, Coulter Counters and flow cytometry technique can also be applied to the analysis of pollen grains in allelopathic studies. They are quite useful in determining the size and number of pollen grains. The technique is often used for assessing the production and size of pollen from the originating individual rather than how much was transferred to heterospecific stigma, as would be needed in a basic assessment of potential allelopathic interactions. 

Durand, R.E., and Olive, P.L. (1983) Flow cytometry techniques for studying cellular thiols. Radiat. Res. 95, 456. 

Analytical flow cytometry offers a rapid and facile means of monitoring cellular receptor content. For example, multiparameter flow cytometry techniques were used to monitor expression of GABAa receptor subunits during neurogenesis in embryonic rat brain (Marie et al., 2001). The content of the cell surface p75 neurotrophin receptor was measured in a heterogeneous population of mouse dorsal root sensory neurons, from which high and low p75 subsets were subsequently isolated by cell sorting (Barrett et al., 1998). 

Light, fluorometric, electronic emission, and confocal absorption microscopy techniques or their combinations with immunometric techniques are applicable to analysis of allergenic compounds regarding the structure of a product—its matrix). Cells and metabolites produced by the immune system cells involved in the allergenic response (specific types of lymphocytes) can be examined by flow cytometry techniques in addition to traditional microscopic techniques. 

Among the items that have been measured are vitality, intracellular pH, DNA and RNA content, and specific plasmids [77,408]. Besides nucleic acids [204], other intracellular components can also be analyzed, e.g. storage materials [2, 82,294], enzymes and protein content [6,338], or the cell size [60,61]. The physiological state can also be rapidly assessed [331]. Furthermore, this technique allows the separation of certain cells using a cell sorter, e.g. for strain improvement [28]. The flow cytometry technique has also been used in connection with molecular probes for identification and viability determination of microbial communities [98]. This application of viability estimation is becoming increasingly important [63, 136, 188, 454]. Unfortunately, the equipment is expensive and most of the measurements are tricky and laborious and not well designed for on-line application. 

Knowledge of these factors coupled to high-resolution microscopy and flow cytometry techniques would allow observation of such short-lived processes as syngamy and meiosis in cultures and in the field. 

We had previously tested that the cell-permeable fluorescent probe diaminofluorescein/diacetate (DAF-2/DA) could be used with the flow cytometry technique to detect intracellular NO [28]. A dose-dependent increase in the intracellular fluorescence of DAF-2 was detected in BAEC treated with CsA (Figure I, right panel). When the accumulation of NO was evaluated in the supernatants of BAEC upon treatment with CsA, a dose-dependent increase in the accumulation of extracellular nitrite was detected (Figure 1, left). As shown in this figure, the concentration of CsA of 1 pM, which lies within the therapeutic range, only produced a moderate increase of NO. 

Flow cytometry Technique for characterizing or. separating particles such as beads or cells, often on the ba.sis of their fluorescence. Used to separate beads that have biologically active molecules attached. 

Other groups treat filters with trypsin/EDTA (10 min, 37°C) prior to quantitating detached cells using flow cytometry techniques. In both these cases, there is considerable variability in the data obtained, and several replicates should be used. 

Flow Cytometry Technique for separating and counting cells or chromosomes by suspending them in fluid and passing them by a focused light. 

Vanderbyl, S., MacDonald, N., and de Jong, G., A flow cytometry technique for measuring... 

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