Florman

Florman, H. M., and Wassarman, P. M. (1985). O-linked oligosacharides of mouse egg ZP3 account for its sperm receptor activity. Cell 41 313-324. 

Florman, Samuel C., The Existential Pleasures of Engineering, St. Martin s Press, 1976. 

Lopez-Gonzalez I, De La Vega-Beltran JL, Santi CM, Florman HM, Felix R, Darszon A (2001) Dev Biol 236 210... 

Paramesh AS, Grosskreutz C, Florman SS, Gondolesi GE, Sharma S, Kaufman SS, Fishbein TM.Thrombotic microangiopathy associated with combined sirolimus and tacrolimus immunosuppression after intestinal transplantation. Transplantation 2004 77 129-131. 

Amoult, C., Zeng, Y., and Florman, H.M. (1995). ZP3-dependent activation of sperm cation channels regulates acrosomal secretion during mammalian fertilization. J. Cell Biol. 134 637-645. 

Florman, H.M. and Babcock, D.F. (1991) Progress towards understanding the molecular basis of capacitation. In CRC Uniscience-Chemistry of Fertilization. (Wassarman, P., Ed.), pp. 105-132. CRC Press Inc. Boca Raton, FL. 

Zeng, Y, Oberdorf J.A., and Florman, H.M. (1996). pH regulation in mouse sperm. Identification of Na, Cl, and HCOj -dependent and arylaminobenzoate-dependent regulatory rhechanisms and characterization of their role in sperm capacitation. Dev. Biol. 773 510-520. 

Harvey M. Florman, Christophe Arnoult, Imrana G. Kazam, Chungqing Li, and Christine M.B. O Toole... 

The participation of G proteins in ZP3 signaling was indicated initially by the inhibition of Ca mobilization (Florman etal., 1989,1992 Bailey etal., 1994) and of acrosome reactions (Endo et al., 1987, 1988) following treatment of intact sperm with pertussis toxin, and subsequently by the direct demonstration of G protein activation by ZP3 in digitonin/cholate-solubilized extracts of sperm (Ward et al., 1992 Wilde et al., 1992 Ward and Kopf, 1993). G, and G j are selectively activated by ZP3 and can account for the pertussis toxin-sensitivity of signaling pathway (Ward... 

This cation channel is activated at an early step in ZP3 signal transduction, as indicated in pharmacological studies (Florman, 1994 Arnoult et al., 1996a). Channel activation produces a small ( 50 nM), transient (1-2 min duration) elevation of Ca i that is restricted to the sperm head. However, this modest rise is not sufficient to initiate acrosome reactions (Florman, 1994). A more plausible role for this channel is to control sperm membrane potential (V ) during ZP3 signal transduction. 

T channel activation is required for ZP3-evoked Ca + elevations and for acrosome reactions. As discussed, fluorescent indicators report that ZP3 stimulation produces a sustained elevation of Ca j in sperm. It is unlikely that inward Ca + current through the T channel can completely account for this response. T channels exhibit rapid, voltage-dependent inactivation and do not produce sustained current (Armstrong and Matteson, 1985 Bean, 1985 Hille, 1992 Bean and McDonough, 1998). In fact, the time resolution of the systems utilized to record ZP3-dependent rises in Ca i are too slow to report T channel activation (Florman et al., 1989 Arnoult et al., 1996b). 

The ZP3-dependent cation channel, an essential component of the signaling cascade, cannot be activated in uncapacitated sperm. Ligand sensitivity is acquired as an early event in capacitation (Florman, 1994). 

Amoult, C., Zeng, Y., and Florman, H.M. (1996a). ZP3-dependent activation of sperm cation channels regulates acrosomal secretion during mammalian fertilization. J. Cell Biol. 754 637-645. Amoult, C., Cardullo, R.A., Lemos, J.R., and Florman, H.M. (1996b). Egg-activation of sperm T-type Ca channels regulates acrosome reactions during mammalian fertilization. Prt>c. Natl. Acad. Sci. 95 13004-13009. 

Amoult, C., Villaz, and Florman, H.M. (1998). Pharmacological properties of the T-type calcium current of mouse spermatogenic cells. Mol. Pharmacol. 55 1104-1111. 

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