Fixed-angle centrifuge

Du Pont RC5C-plus fixed-angle centrifuge... 

Benchtop centrifuge lEC Model HN-SII 6 x 50-mL fixed-angle solid rotor, 15-mL adapter sleeves Beckman pH meter... 

Free subcellular particles or virus from Percoll by centrifuging the respective fraction of cell fractionation in a fixed-angle rotor at 100 000 X g for 90 min. Percoll forms a solid pellet whidi contains practically no material of interest. 

Most laboratories have a standard low-speed centrifuge used for routine sedimentation of relatively heavy particles. The common centrifuge has a maximum speed in the range of 4000 to 5000 rpm, with RCF values up to 3000 X g. These instruments usually operate at room temperature with no means of temperature control of the samples. Two types of rotors, fixed angle and swinging bucket, may be used in the instrument. Centrifuge tubes or bottles that contain 12 or 50 mL of sample are commonly used. Low-speed... 

Rotors for a high-speed centrifuge. A Fixed angle B swinging bucket C vertical. Courtesy of Beckman Instruments, Inc. 

Operation of a fixed angle rotor A Loading of sample B Sample at start of centrifugation C Bands form as molecules sediment D Rotor at rest showing separation of two components... 

Figure 10.5 Pore water extraction by (A) drainage centrifugation using a swing-out rotor and (B) immiscible liquid displacement using a fixed-angle rotor (after Kinniburgh and Miles, 1983).

Thermostatized centrifuge with fixes angle rotor (angle of inclination 33°) (e.g. Hettich Rotixa RP 001260 Tuttlingen Germany)... 

It is supplied with a dual purpose head. The buckets can either swing out to the horizontal position or, by means of a rubber adapter, the buckets can be held at a fixed angle of 45° (M.S.E.) or 51° (International) in the latter case, the instrument acts as an angle centrifuge. This is an alternative to the hand centrifuge. 

Figure 4-36. Fixed angle rotor. On the left is illustrated a conventional centrifuge tube, in which liquid is pressed against the lid during centrifugation. The right depicts a tube with an offset neck to avoid this problem.

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