Filamentous fungi strain

For example, a screening of 416 strains (71 bacterial strains, 45 actinomycetes, 59 yeast, 60 basidiomycetes, 33 marine fungi, and 148 filamentous fungi) has been performed to look for microorganisms that display reductase activity in the absence of oxidase activity [8b]. A new microorganism, Diplogdasinospora grovesii IMI... 

Pectin degradation requires fee combined action of various enzymatic activities. However, evaluation of fee contribution of individual pectinases in Suit juice extraction and clarification is rather complicated. Most commercial pectinolytic enzyme preparations are produced by fermentation wife filamentous fungi, mostly strains belonging to fee genus Aspergillus,. plication studies with mixtures of isolat enzymes obtained by fermentation or by means of fractionation of commercial enzyme preparations can be used to assess the importance of fee various individual enzymes. Subsequently, molecular biology and fermentation technology can be used to enhance specific desirable enzymatic activities. 

Very recently, in 1999, an European patent was published involving the description of a pharmaceutical composition, which includes terpenoid dilactones isolated from a new strain of Oidiodendrum griseum filamentous fungi, together with some semi-synthetic derivatives from the isolated natural podolactones [7]. This pharmaceutical composition was reported to be useful for the treatment of IL-1 (interleukin-1) and TNF (tumor necrosis factor)-mediated diseases. 

Index Entries Alcohol oxidase filamentous fungi hydrocarbon biodegradation petroleum contamination YR-1 strain. 

The filamentous fungi screened were all from the genus Penicillium (Table 1) and were selected from the culture collection at BioCentrum-DTU, Technical University of Denmark. The filamentous fungi T. reesei Rut C30 was used as reference strain. 

Adapted media for the microbial strains potato dextrose broth (PDB, Difco) used at half-strength (12 g/L for filamentous fungi supplemented with antibiotics) and Poor broth (PB, 1% bactotryptone, 0.9% NaCl w/v, pH 7.4) if bacterial strains for activity screening during isolation. 

Besides addition of heme, the influence of culture temperature on heterologous production of peroxidases has also been reported. For example, lowering the culture temperature from 28 to 19°C enhanced the level of active versatile peroxidase of P. eryngii 5.8-fold and reduced the effective proteolytic activity of the A. nidulans host strain by 2-fold. In this way, a maximum peroxidase activity of 466 U/L was reached [42]. Efficient heterologous production of peroxidases is not always dependent on the availability of heme. The heterologous production of Arthromyces ramosus peroxidase (ARP) has been analyzed in A. awamori under the control of the inducible endoxylanase promoter. Secretion of active ARP was achieved at up to 800 mg/L in shake flask cultures without addition of hemin [43]. This represents a 1,600-fold increase in production compared to ARP production in S. cerevisiae and 38-fold increase compared to ARP production in P. pastoris (see Sect. 12.2). These observations support that several filamentous fungi are more effective secretors of proteins than yeast strains like S. cerevisiae and P. pastoris. Also for... 

The expression of nitrilase may be enhanced and, thus, screening for its activity fadhtated by the choice of an appropriate nitrilase inducer. For instance, in five strains belonging to three fungal genera 2-cyanopyridine enhanced the nitrilase activity by two to three orders of magnitude. Therefore, this nitrilase inducer appears to be very efficient and of wide use in filamentous fungi [25]. 

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