Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Fibroblasts forces

FEV forced expiratory volume, fibroblast connective tissue cell. [Pg.413]

This involves the use of a special rotor and the adaptation of the centrifuge for constant flow as for the zonal rotor. This is expensive, but once installed, reproducible separations of 108-109 cells can be achieved in under an hour and cells of all sizes (rather than just the smallest) are obtained. It is particularly suited to the isolation of Gl-phase cells from suspension cultures for which mitotic detachment procedures are not applicable. The separation chamber is kite-shaped with the buffer solution entering at the acute point on the rim of the centrifuge and leaving at the obtuse point towards the centre of rotation (Fig. 11.2). Thus the centrifugal forces on particles within the chamber are countered by the centripetal flow of buffer and particles come to equilibrium within the chamber. When equilibrium has been reached, the samples are pumped out, by increasing the rate of buffer flow, and collected. Meistrich et al. (1977) obtained 3 fractions of L-P59 mouse fibroblasts which were over 90% Gl-phase, 70% S-phase cells and 60% G2 + M-phase cells, respectively. [Pg.219]

Temin (1970) and Todaro et al. (1965) showed similar effects for chicken fibroblasts and 3T3 mouse fibroblasts. The low level of serum is important for survival as well as for the subsequent stimulation of DNA synthesis (Cherrington, 1984). A kinetic analysis using time lapse cinematography (Zetterberg and Larsson, 1985) showed that Swiss 3T3 cells were only susceptible to cell cycle arrest in a short period (3-4 h) following mitosis. Even a 1-h exposure to serum-free medium during this time forced the cells into GO from which they required 8 h to return to Gl. The length of the postmitotic sensitive phase was very constant at between 3 and 4 h but considerable intercellular variability existed in the duration of the pre S-phase Gl period consistent with a transition probability event ( 10.4). [Pg.225]

ECMs contain a variety of cell types (see Table 1.2) such as fibroblasts in the skin, chondrocytes in cartilage, and osteoblasts in bone. The role of these cells is to synthesize and deposit the ECM surrounding the cell and to change the amount and location of the ECM in response to trauma and changes in external loading. For instance, collagen synthesis in these cells is typically up-regulated by the application tensile forces and is down-... [Pg.5]

Fibroblasts grown in collagen lattices can generate a force of approximately 10 x 10 WN as a result of a change in cell shape and attachment they maintain a tensional homeostasis of approximately 40-60 x 10 5N per... [Pg.238]

Eastwood M, McGrouther DA, Brown RA. Fibroblast responses to mechanical forces. Proc Inst Mech Eng. 1998 212 85-92. [Pg.252]


See other pages where Fibroblasts forces is mentioned: [Pg.78]    [Pg.80]    [Pg.86]    [Pg.86]    [Pg.130]    [Pg.182]    [Pg.414]    [Pg.40]    [Pg.205]    [Pg.353]    [Pg.101]    [Pg.94]    [Pg.100]    [Pg.106]    [Pg.113]    [Pg.70]    [Pg.134]    [Pg.128]    [Pg.169]    [Pg.11]    [Pg.21]    [Pg.21]    [Pg.21]    [Pg.84]    [Pg.93]    [Pg.224]    [Pg.226]    [Pg.231]    [Pg.237]    [Pg.238]    [Pg.238]    [Pg.239]    [Pg.239]    [Pg.245]    [Pg.126]    [Pg.133]    [Pg.7]    [Pg.77]    [Pg.1240]    [Pg.146]    [Pg.205]    [Pg.88]    [Pg.67]    [Pg.257]    [Pg.155]   
See also in sourсe #XX -- [ Pg.237 , Pg.238 , Pg.239 ]




SEARCH



Fibroblasts

© 2024 chempedia.info