Fatty acids hydrogen peroxide formation

Where does the hydrogen peroxide originate Many metabolic processes produce hydrogen peroxide as a byproduct. In particular, the degradation of amino acids and fatty acids leads to hydrogen peroxide formation. 

It is not clear why LA and none of the saturated fatty acids that were studied disrupted endothelial barrier function. The injurious effects of LA on cultured endothelial cells may be mediated, in part, by the induction of peroxisomes and, thus, by excessive hydrogen peroxide formation. In addition, enrichment of endothelial lipids with selective fatty acids can modify specific cellular lipid pools and alter the morphology of cultured cell monolayers. Such fatty acid-mediated compositional changes may be sufficient to alter membrane properties, e.g., fluidity and activities of membrane-bound enzymes. One may speculate from these and other data that high dietary intakes of certain unsaturated fatty acids, such as LA, might not be entirely safe. 

The basis for all enzymatic cholesterol assays is the hydrolysis of cholesterol esters by cholesterol esterase (CEH, EC 3.1.1.13) to free cholesterol and fatty acids and the oxidation of free cholesterol to cholestenone by cholesterol oxidase (COD, EC 1.1.3.6) with concomitant oxygen consumption and hydrogen peroxide formation ... 

These results show that erucate and other Q2 fatty acids to a great extent depend on the peroxisomes for their normal metabolism. The acids are shortened by one or two P-oxidation cycles in the peroxisomes and then transferred to the mitochondria for complete oxidation. The adaptation to the presence of these fatty acids in the diet is the result mainly of an increased peroxisomal P-oxidation capacity which means an increased shortening capacity. This adaptation also means an increased rate of hydrogen peroxide formation in the tissues. In accordance with these results Foerster et al. [8] have shown in perfused livers that erucic acid increases the formation of hydrogen peroxide. Palmitate had no effect indicating that this acid normally is oxidized almost exclusively in the mitochondria. The role of peroxisomes in the oxidation of the very-... 

The mechanism of inhibition by the salts of the long chain fatty acids has been examined . It was concluded that, in the case of the lead salts, metallic lead was first deposited at certain points and that at these points oxygen reduction proceeded more easily, consequently the current density was kept sufficiently high to maintain ferric film formation in addition, any hydrogen peroxide present may assist in keeping the iron ions in the oxide film in the ferric condition, consequently the air-formed film is thickened until it becomes impervious to iron ions. The zinc, calcium and sodium salts are not as efficient inhibitors as the lead salts and recent work has indicated that inhibition is due to the formation of ferric azelate, which repairs weak spots in the air-formed film. This conclusion has been confirmed by the use of C labelled azelaic acid, which was found to be distributed over the surface of the mild steel in a very heterogeneous manner. ... 

These include the mitochondrial respiratory chain, key enzymes in fatty acid and amino acid oxidation, and the citric acid cycle. Reoxidation of the reduced flavin in oxygenases and mixed-function oxidases proceeds by way of formation of the flavin radical and flavin hydroperoxide, with the intermediate generation of superoxide and perhydroxyl radicals and hydrogen peroxide. Because of this, flavin oxidases make a significant contribution to the total oxidant stress of the body. 

Oxidation to CO of biodiesel results in the formation of hydroperoxides. The formation of a hydroperoxide follows a well-known peroxidation chain mechanism. Oxidative lipid modifications occur through lipid peroxidation mechanisms in which free radicals and reactive oxygen species abstract a methylene hydrogen atom from polyunsaturated fatty acids, producing a carbon-centered lipid radical. Spontaneous rearrangement of the 1,4-pentadiene yields a conjugated diene, which reacts with molecular oxygen to form a lipid peroxyl radical. 

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