Fatty acid amidohydrolase

Once inside the cell, anandamide is hydrolyzed by a specific hydrolase, anandamide amidase (AEAase) or fatty acid amidohydrolase (FAAH) (Desarnaud, 1995 Deutsch, 1993). This enzyme is membrane associated and shows significant specificity for anandamide (Desarnaud, 1995 Lang, 1999). [Pg.109]

Steffens M, Schulze-Bonhage A, Surges R, Feuerstein TJ (2005) Fatty acid amidohydrolase in human neocortex-activity in epileptic and non-epileptic brain tissue and inhibition by putative endocannabinoids. Neurosci Lett 385 13-17... [Pg.23]

Reggio PH, Traore H (2000) Conformational requirements for endocannabinoid interaction with the cannabinoid receptors, the anandamide transporter and fatty acid amidohydrolase. Chem Phys Lipids 108 15-35... [Pg.76]

Fowler CJ, Tiger G, Lopez-Rodriguez ML, Viso A, Ortega-Gutierrez S, Ramos JA (2003) Inhibition of fatty acid amidohydrolase, the enzyme responsible for the metabolism of the endocannabinoid anandamide, by analogues of arachidonoyl-serotonin. J Enzyme Inhib Med Chem 18 225-231... [Pg.177]

Holt S, NUsson J, Omeir R, Tiger G, Fowler CJ (2001) Effects of pH on the inhibition of fatty acid amidohydrolase by ibuprofen. Br J Pharmacol 133 513-520... [Pg.204]

Vandevoorde, S., Lambert, D.M., Smart, D., Jonsson, K.O., and Fowler, C.J. (2003) N-Morpholino- and N-diethyl-analogues of palmitoylethanolamide increase the sensitivity of transfected human vanilloid receptors to activation by anandamide without affecting fatty acid amidohydrolase activity, Bioorg. Med. Chem., 11 817-25. [Pg.174]

CBj - Neuronal cannabinoid receptor CBj - Peripheral cannabinoid receptor FAAH - Fatty acid amidohydrolase... [Pg.445]

Lopez-Rodriguez, M.L., Viso, A., Ortega-Gutierrez, S., Fowler, C.J., Tiger, G., de Lago, E. et al. (2003) Design, synthesis, and biological evaluation of new inhibitors of the endocannabinoid uptake comparison with effects on fatty acid amidohydrolase. Journal of Medicinal Chemistry 46 1512-1522. [Pg.464]

Zafiriou PM, Karava V, Boutou E, Vorigas CE, Maccarrone M, Siafaka-Kapadai A. Partial purification and characterization of a fatty acid amidohydrolase (FAAH) from Tetrahymena pyriformis. 2004 Annual Symposium on the Cannabinoids, International Cannabinoid Research Society, Burlington VT, p. 146. [Pg.513]

Maurelli S, Bisogno T, De PetrocelUs L, Di Luccia A, Marino G, Di Marzo V (1995) Two novel classes of neuroactive fatty acid amides are substrates for mouse neuroblastoma anandamide amidohydrolase . FEBS Lett 377 82-86... [Pg.22]

Ueda N, Yamamoto S (2000) Anandamide amidohydrolase (fatty acid amide hydrolase). Prostaglandins Other Lipid Mediat 61 19-28... [Pg.598]

The actual enzymes involved in fatty acid amides (FAAs) metabolism remained unknown until the late 1990s, when a rat liver oleamide hydrolase activity was affinity-purified and its cDNA was cloned (Cravatt et al., 1996). Oleamide amidase was connected to AEA hydrolysis, because AEA and oleamide were catalyzed by the same enzyme, called fatty acid amide hydrolase (FAAH A-arachidonoylethanolamine amidohydrolase, EC 3.5.1.4) (Maurelli et al.,... [Pg.109]

Anandamide amidohydrolase / Fatty acid amide hydrolase... [Pg.185]

Gerwick et al. (1997) described an enzyme from the red alga Ptilota filicina, which they named polyenoic fatty acid isomerase. The enzyme was capable of converting AEA into novel substances such as conjugated triene anandamide. Chapman (2000) described FAAH-like amidohydrolase... [Pg.524]

The discovery of anandamide (arachidonoyl ethanolamide, AEA) and of its manifold roles in the central nervous system and in the periphery (reviewed in refs. 1 and 2) prompted several researchers to develop analytical methods to assay and characterize the activity of the enzymes responsible for AEA metabolism in various cells and tissues. Fatty acid amide hydrolase (arachidonoyl ethanolamide amidohydrolase, EC 3.5.1.4 FAAH) has emerged as the key AEA hydrolase, showing a molecular mass of approx 64 kDa and an optimum pH of around 9.0 (3). Recently, FAAH has been crystallized,and its three dimensional structure has been determined at 2.8A resolution (4). This enzyme cleaves the amide bond and releases arachidonic acid and ethanolamine. High-performance liquid chromatography (HPLC) is the most widely used method to determine FAAH activity from different sources. We developed a new method (5) based on reversed-phase (RP)-HPLC and on-line scintillation counting, which combines the need for high resolution, reproducibility, and sensitivity... [Pg.163]

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