Fast liquid chromatography versatility

Reverse phase liquid chromatography is very versatile, fast and highly reproducible. Aqueous solutions are normally used and the modifiers used are very cheap and highly pure. Separation is predictable based on the polarity, pH profile, solubility and other physicochemical characteristics of the solute molecules. Analysis time is rather short and reequilibration is generally fast. Multiple components with minor differences in polarity can be separated by appropriate choice of gradient profiles. 

Further advances have resulted from developments in instrumentation, particularly in the areas of scanning densitometry and automated sample application, which have now made fully instrumental quantitative TLC a reality far removed from the basic practice. TLC is now regarded as an indispensable tool in both quality control and research laboratories. The technique is easy to learn and is fast and versatile and in many instances may be preferred to the techniques of gas chromatography and high performance liquid chromatography. 

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