Fab display

Ames RS, Tornetta MA, Jones CS, Tsui P, Isolation of neutralizing anti-C5a monoclonal antibodies from a filamentous phage monovalent Fab display library, J. Immunol., 152 4572-4581, 1994. 

Whatever the ionization method used, the mass spectra of oligosaccharides analysed by ESI, MALDI or FAB display intense ions of the molecular species resulting from protonation (M -(- H)+ or cationization by an alkali metal ion (M + alkali metal)"1" in the positive ion mode or from deprotonation (M — II) in the negative ion mode. In ESI, multiply charged ions also are produced. 

RS Ames, MA Tometta, LJ McMillan. Neutralizing murine monoclonal antibodies to human IL-5 isolated from hybridomas and a filamentous phage Fab display library. J Immunol 1545 6355-6364, 1995. 

Various antibody preparations have been developed that facilitate imaging of vascular-related conditions, including myocardial infarction, deep vein thrombosis and atherosclerosis. Anti-myosin monoclonal antibody fragments (Fab) labelled with mIn, for example, have been used for imaging purposes in conjunction with a planar gamma camera. The antibody displays specificity for intracellular cardiac myosin, which is exposed only upon death of heart muscle tissue induced by a myocardial infarction (heart attack). 

Library A collection of antibodies, usually Fab or scFv fragments, in the range of 106 to 1010 and displayed on the surface of bacteriophage whose DNA gene contains a DNA sequence capable of expression as the antibody protein. Thus, identification of a single member of the library by selection can be used to generate multiple copies of the phage and sizeable amounts of the antibody protein. 

Phenomenological chiral discrimination of various permethylated linear oligosaccharides toward organic amines has been examined by using the FAB-MS-EL guest method." " " As shown in Table 15, permethylated fructo-oligosaccharides, especially the permethylated 1 -fructonystose (MeFruNys in Scheme 8), display a remarkable chiral discrimination ability." If compared to linear... 

Figure 21 Hapten 35 and fluorogenic substrates 36-38 used to screen phage displayed Fabs with an aldolase activity.

Depending on the particular vector system used, gp3 fusions can be displayed either multivalently for selection by avidity or monovalently to select for binders with the highest affinity. By fusing VH or VL antigen binding domains (Fv) or VH-CH and VL-CL (Fabs) to a bacterial leader sequence, the Ab chains are directed through the cytoplasmic membrane into the gap between... 

A number of phagemid display systems that fuse Ab chains to either full-length or N-terminally deleted gp3 fusions have been described, along with the polymerase chain reaction (PCR) primer sets, restriction enzymes, and host strains required for the display of scFvs and Fabs (3—7). Some systems are commercially available. There is an important difference in whether the Ab-gp3 fusions are with the whole of the protein or with just the C-terminal domain. Fusions to the whole of gp3 require that the expression of the fusion is suppressed until after it is infected with the helper phage because of the resistance... 

Hoogenboom, H. R., Griffiths, A. D., Johnson, K. S., Chiswell, D. J., Hudson, P., and Winter, G (1991) Multi-subunit proteins on the surface of filamentous phage, methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res 19,4133-4137... 

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