Phagemid display

A number of phagemid display systems that fuse Ab chains to either full-length or N-terminally deleted gp3 fusions have been described, along with the polymerase chain reaction (PCR) primer sets, restriction enzymes, and host strains required for the display of scFvs and Fabs (3—7). Some systems are commercially available. There is an important difference in whether the Ab-gp3 fusions are with the whole of the protein or with just the C-terminal domain. Fusions to the whole of gp3 require that the expression of the fusion is suppressed until after it is infected with the helper phage because of the resistance... 

Phage and phagemid display Some important parameters... 

NOMENCLATURE FOR M13 TYPE PHAGE- AND PHAGEMID-DISPLAY VECTOR TYPES AS PROPOSED BY SMITH [209]... 

Bacteriophage fd414, 416 < X174 391 M13 414,416 phage display 416-418 phagemid 416 Bamase... 

FIGURE 16.4 Schematic representation of phage (b) and phagemid (c) display of polypeptides fused to pill and pVIII coat proteins, compared to wild-type phage (a). 

The one-gene system display (phage) is a good system for the display of small peptides, which do not alter the structure of the coat protein and do not prevent its assembly into viral particles. For the display of larger peptides the two-gene system (phagemid) is preferred or sometimes required, as for pVIII fusion. 

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