Extracellular fluid microdialysis

Figure 4.8 Noradrenaline concentration in dialysis samples from probes implanted in the rat frontal cortex. Spontaneous efflux of noradrenaline is stable throughout a 4h sampling period ( extended basals ) but is increased markedly when either the noradrenaline reuptake inhibitor, desipramine (5 pM), or the a2-adrenoceptor antagonist, atipamezole (0.5 pM), is infused into the extracellular fluid via the microdialysis probe ( retrodialysis )...

The technique consists of a microdialysis probe, a thin hollow tube made of a semi-permeable membrane usually around 200-500 /xm in diameter, which is implanted into the skin and perfused with a receiver solution that recovers the unbound permeant from the local area. In principle, the driving force of dialysis is the concentration gradient existing between two compartments separated by a semi-permeable membrane. For skin under in vivo conditions, these compartments represent the dermal or subcutaneous extracellular fluid (depending on the probe position) and an artificial physiological solution inside the probe [36-38],... 

In Vivo Microdialysis A Method for Sampling Extracellular Fluid in Discrete Brain Regions... 

In vivo microdialysis a method for sampling extracellular fluid in discrete brain regions... 

McNay EC, Sherwin RS. 2004. From artificial cerebro-spinal fluid (acsf) to artificial extracellular fluid (aecf) microdialysis perfusate composition effects on in vivo brain ecf glucose measurements. J Neurosci Methods 132(1) 35-43. 

Yadid G, Pacak K, Kopin IJ, Goldstein DS. 1993. Modified microdialysis probe for sampling extracellular fluid and administering drugs in vivo. Am J Physiol 265(5 Pt 2) R1211-R1211. 

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