Experimental methodology local control

Although the FIFRA microcosm has a number of advantages, there are also compromises. The few experiments that have been conducted and the variance in methodologies have not provided an accurate representation of the repeatability or replicability of the experiments. In addition, the method is somewhat local specific since the temperature, diurnal cycle, and to some extent the experimental organisms are controlled by the local environmental conditions. On the other hand, the sensitivity to local conditions can also act as a more accurate model of local fate and effects of the test material. 

In this section, the question raised above will be addressed by applying the local control methodology to a two-dimensional quantum model of the active site of hemoglobin. This system has been studied extensively for many years, both theoretically and experimentally, using a wide range of theoretical methods or experimental techniques. For a recent review of the theoretical approaches, see Ref. 144 and references therein. Here, we choose the six-coordinated iron-porphyrin-imidazole-CO (FeP(Im)-CO) as active site model, which recently has also been used for the study of the excited states [145]. In this complex, the imidazole mimics the proximal histidine, which binds to the central Fe atom and a second imidazole is placed in the proximity of the complex, to include the influence of the distal histidine. The respective configuration is sketched in Fig. 8. 

In the present study, we show an example of the application of this experimental methodology to the investigation of adsorption characteristic of local bentonite clay. Adsorption / desorption experiment were performed at laboratory scale with model-contaminated gaseous stream containing o-xylene that has been selected as rep resentative VOC because it is environmentally relevant regarding industrial concern. The work aimed to help further development of low cost materials involved in environmental engineering control. 

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