Exocrine Pancreatic Secretion in Anesthetized Dogs

The duct system of the canine pancreas is different from that of the human pancreas. In dogs, the main duct is the accessory pancreatic duct (Ductus pancre- 

the pancreatic fistula technique in anesthetized dogs is described. The effect of exogenous hormones, e.g. secretin or gastrin, or of vagal stimulation, on exocrine pancreatic secretion can be measured in anesthetized dogs with acute pancreatic fistulas. 

At the end of each collection period, the volume is recorded, and the bicarbonate content determined titri-metrically. Furthermore, pancreatic enzymes, such as amylase, are determined in the samples. Determination of protein concentrations in the pancreatic juice can be used as end-point since the total protein concentration is proportional to the individual enzymes (Keller et al. 1958). In a pre-test period of 10 min, samples are collected every 2 min. Then, the test compound is injected intravenously and the pancreatic juice is collected every 2 min. 

The secretion after injection of the test compound is compared with the pre-test values. Secretin increases pancreatic volume and bicarbonate secretion in a dose-dependent manner and can be ideally used as reference secretagogue. 

Glad et al. (1996) tested the influence of gastrinreleasing peptide on acid-induced secretin release and pancreatobiliary and duodenal bicarbonate secretion in Danish country strain pigs weighing between 22 and 30 kg. The animals, starved overnight with free 

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