Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Evaporative light scattering problems

Thin-layer chromatography (TLC) is used both for characterization of alcohol sulfates and alcohol ether sulfates and for their analysis in mixtures. This technique, combined with the use of scanning densitometers, is a quantitative analytical method. TLC is preferred to HPLC in this case as anionic surfactants do not contain strong chromophores and the refractive index detector is of low sensitivity and not suitable for gradient elution. A recent development in HPLC detector technology, the evaporative light-scattering detector, will probably overcome these sensitivity problems. [Pg.283]

Detectability may be a significant problem with homologous series of unsaturated compounds, particularly //-alkanes. For these compounds, refractive index detection or evaporative light-scattering, both of which are described elsewhere in the book, may be of use. Indirect photometry is a useful detection scheme for compounds that do not absorb in the UV. Acetone, methylethyl ketone, methyl propyl ketone, methyl isopropyl ketone, methyl isobutyl ketone, and acetophenone are added to an acetonitrile/water mobile phase, generating a negative vacancy peak when the nonchro-mophoric analyte emerges and a positive peak if the ketone is adsorbed and displaced.70 Dodecyl, tetradecyl, cetyl, and stearyl alcohols also have been derivatized with 2-(4-carboxyphenyl)-5,6-dimethylbenzimidazole and the derivatives separated on Zorbax ODS in a mobile phase of methanol and 2-propanol.71... [Pg.161]

Detection is also frequently a key issue in polymer analysis, so much so that a section below is devoted to detectors. Only two detectors, the ultra-violet-visible spectrophotometer (UV-VIS) and the differential refractive index (DRI), are commonly in use as concentration-sensitive detectors in GPC. Many of the common polymer solvents absorb in the UV, so UV detection is the exception rather than the rule. Refractive index detectors have improved markedly in the last decade, but the limit of detection remains a common problem. Also, it is quite common that one component may have a positive RI response, while a second has a zero or negative response. This can be particularly problematic in co-polymer analysis. Although such problems can often be solved by changing or blending solvents, a third detector, the evaporative light-scattering detector, has found some favor. [Pg.333]

Detection in 2DLC is the same as encountered in one-dimensional HPLC. A variety of detectors are presented in Table 5.2. The choice of detector is dependent on the molecule being detected, the problem being solved, and the separation mode used for the second dimension. If MS detection is utilized, then volatile buffers are typically used in the second-dimension separation. Ultraviolet detection is used for peptides, proteins, and any molecules that contain an appropriate chromophore. Evaporative light scattering detection has become popular for the analysis of polymers and surfactants that do not contain UV chromophores. Refractive index (RI) detection is generally used with size exclusion chromatography for the analysis of polymers. [Pg.109]

The advent of the use of mass spectrometers as detectors and new mass detectors such as the charged aerosol detectors (CAD) and evaporative light scattering detectors (ELSD) should provide high-sensitivity detection of compounds that do not absorb UV light. The only problem with most of these is that they are expensive and, therefore, not readily available. When prices come down, they should finally eliminate the use of derivatives in HPLC analysis. [Pg.151]

The detection problems of the LC LC procedmes are similar to LC CC (see section 11.8.3). The zone of interacting tnacromolectrles is partially overlapped with the barrier and this fact complicates quantitative sample monitoring. This is why the evaporative light scattering detectors with all their drawbacks (see section 11.6.1.4) are so far mostly employed in the LC LC measurements. [Pg.319]

The use of diode-array detection is limited because many of the terpenoids do not have chromophores for the UV region. Refractive index, evaporative light scattering detectors (ELSD), or low-UV detectors can overcome, to a certain extent, this problem. However, the refractive index detector... [Pg.2296]


See other pages where Evaporative light scattering problems is mentioned: [Pg.260]    [Pg.165]    [Pg.349]    [Pg.500]    [Pg.79]    [Pg.188]    [Pg.14]    [Pg.1436]    [Pg.1593]    [Pg.22]    [Pg.119]    [Pg.13]    [Pg.448]    [Pg.2710]    [Pg.41]    [Pg.46]    [Pg.301]    [Pg.1243]    [Pg.572]    [Pg.1045]    [Pg.1071]    [Pg.1603]    [Pg.57]    [Pg.55]    [Pg.4927]    [Pg.829]    [Pg.837]    [Pg.1364]    [Pg.1521]    [Pg.107]    [Pg.345]    [Pg.354]    [Pg.155]    [Pg.519]    [Pg.1308]    [Pg.447]    [Pg.485]    [Pg.512]    [Pg.55]    [Pg.360]    [Pg.125]   
See also in sourсe #XX -- [ Pg.393 ]




SEARCH



Evaporative light scattering

© 2024 chempedia.info