Evans blue dye

Fig. 13.1 Cytoprotective action of adenosine in a quantitative model of mouse hindlimb ischemia and reperfusion (I/R) injury. Adult wild type mice were injected with (a) sterile vehicle (0.1% DMSO in phosphate-buffered saline, pH 7.4) or various adenosine receptor agonists (b, c) or antagonist (d, f). (a) Following ischemia and reperfusion, skeletal muscle showed a significant uptake of Evans Blue dye (EBD) in vehicle-treated mice (first part of (a), representative of 7 mice). The contra-lateral leg not subjected to ischemia-reperfusion showed virtually no EBD uptake. In the second part of (a), the same section was stained with rabbit polyclonal anti-skeletal muscle actin antibodies followed by staining with goat anti-rabbit IgG conjugated with FITC. (b) The nonselective adenosine agonist R-PIA caused a large reduction in the EBD-stained area (see both first and second parts to this figure, representative of six mice)...

Figure 8.6 shows a diagram of a general model of blood-tissue solute transport, used to analyze data on the transport of labeled solutes introduced in the blood or perfusate flow supplied to individual organs. The development and analysis of models of this sort to analyze solute transport in physiological systems is a field pioneered by Sangren and Sheppard [178], Renkin [172], and Crone [40], Optically detectable probes (such as Evans Blue dye bound to albumin) can be used in conjunction with model analysis to probe the intravascular transport of... 

SYNS 4,4 -BIS(7-(l -AMINO-8-HYDROXY-2,4-DISULFO)-NAPHTHYLAZO)-3,3 -BITOLYL, TETRASODIUM SALT 4,4 -BIS(l-AMINO-8-HYDROXY-2,4-DISULPHO-7-NAPHTHYLAZO)-3,3 -BITOLYL, TETRASODIUM SALT BLEKIT EVANSA (POLISH) CHLORAZOL SKY BLUE FF C.I. 23860 C.I. DIRECT BLUE 53 DIAMINE SKY BLUE FF DIAZOBLEU DIAZOL PURE BLUE FF DYE EVANS BLUE EB EVABLIN EVANS BLUE DYE GEIGY-BLAU 536 T 1824... 

Figure 5 Diagram of staining of the bronchial circulation in the cat. The bronchial and pulmonary circulations of the cat were perfused separately with aerated physiological salt solution containing bovine serum albumin (4% wt/vol) maintained at 37°C. Perfusates from the bronchial and pulmonary circulations were collected from cannulae positioned in the right and left ventricles, respectively. Infusion of Evans blue dye (30mg/Kg) into the systemic circulation resulted in deep blue staining of the central airways (black) with no staining of the parenchymal tissues (dotted). Further, 75-80% of the dye was collected from the cannula from the right heart.

Lorsgren P, et al. Intrapulmonary deposition of aerosolized Evans blue dye and liposomes in an experimental porcine model of early ARDS. Ups J Med Sci 95(2) 117-136, 1990. 

The dose of Evans blue dye solution was incorrectly listed in the protocol as 0.1 mg/kg the 5 mg/mL stock solution was dosed at 0.1 mlTkg, resulting in an Evans blue dye dose of 0.5 mgdtg. 

Figure 20. Example of the sharp PDT threshold boundary in tissue (in this case, normal liver treated with a second-generation photosensitizer). The surface light irradiation is indicated, and the contour follows the necrosis boundary, defined by Evans Blue dye exclusion.

Prepare the challenge antigen solutions by making 500 pl/ml solutions of PA-PGSA or GPSA in 1.0% Evans blue dye. 

Evans blue Evans blue dye. See Direct blue 53 Evanstab 12. See Dilauryl thiodipropionate Evanstab 13. See Ditridecyl thiodipropionate Evanstab 14. See Dimyristyl thiodipropionate Evanstab 18. See Distearyl thiodipropionate Evanstab 1218. See Stearyl lauryl thiodipropionate... 

Fig. 2. Photographs of the brain setions stained with Evans blue dye. The staining was performed on the brains reperfused for 6 h after administering a control antibody (A) or an anti-IL-8 mAb (B).

The characteristics of vascular pathophysiology Usted in Table 1 contribute to the selective, enhanced accumulation and prolonged retention of macro-molecular drugs or lipid particles in tumor tissue (EPR effect). Evans blue dye, which forms complexes with albimiin (67 kDa) or lipiodol (a lipid contrast agent), clearly revealed the presence of the EPR effect in rodent tumors after intravenous injection of Evans blue bound albumin, or after arterial... 

Serum albumin, mouse Evans blue dye - 2 hr 30 hr Mouse... 

McDonald DM, Blewett RW. Location and size of carotid body-like organs (paragangha) revealed in rats by the peimeahility of hlood vessels to Evans blue dye. J Neurocytol 1981 10 607-643. 

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