Blasticidin-S deaminase

Bsd Blasticidin S deaminase Blasticidin ( . coli and yeast) www.invitrogen.com... 

Yatnaguchi 1, Seto H, Misato T. Substrate binding by blasticidin S deaminase, an aminohy-drolase for novel 4-aminopyrimidine nucleosides. Pestic Biochem Physiol 1986 25 54-62. 

Yamaguchi I, Shibata H, Seto H, Misaco T. Isolation and purification of blasticidin S deaminase from Asper0lius teneus. Antibiot 1975 28 7-14. 

Yamaguchi 1, Misato T. Active center and mode of reaction of blasticidin S deaminase. Agric Biol Chem 1985 49 3355-3361. 

Kobayashi K, Kamakura T, Tanaka T, Yamaguchi I, Endo T. Nucleotide sequence of the bsr gene and N-terminal amino acid sequence of blasticidin S deaminase from blasticidin S resistant Bsclieridub co/i TK121. Agric Biol Chem 1991 55 3155-3157-... 

Kamakura T, Yoneyama K, Yamaguchi I. Expressitxi of the blasticidin S deaminase gene (bsr) in tobacco Fungicide tolerance and a new selective marker for transgenic plants. Mtd Gen Genet 1990 223J32-334. 

Pleomycin [30] and blasticidin S [31, 32] resistance genes are suitable selection markers for gene disruption experiments, as they confer resistance to the antibiotics as single-copy genes. The blasticidin deaminase gene (hsr) is inserted as a single copy only if low concentrations of 5-10 pg mL blasticidin are used for selection. Otherwise, between one and 20 copies per cell have been observed after selection with 10-60 pg blasticidin [24, 28]. 

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