Eugenol Extraction methods

A much more forgiving yet limited extraction method can be used to isolate phenol species such as eugenol and chavicol. You see farther back in this chapter where one can use dilute NaOH to remove eugenol from sassafras oil Well, why not use it to isolate the damn things for further research. It works like a charm ... 

For example, vanillin can be obtained via at least five different ways (i) by isolation from the orchid (Vanilla planifolia), which is a very expensive method (ii) by tissue culture followed by extraction (iii) by microbial transformation of eugenol, the main compound of clove (iv) from lignine by synthesis, and (v) from guaiacol, a natural aroma compound, with comparable molecular structure. Only the vanillin obtained via the first three methods is natural. The other routes afford a nature-identical vanillin. 

Reversed phase isocratic HPLC with ultraviolet detection at 280 nm was used to separate and identify eugenol in the ethanolic extract of whole tobacco and clove cigarettes [25]. The samples were analyzed at 30°C on a RP 18 column using methanol-water (80 20) as the mobile phase. This method was also applied to determine the eugenol content within the total particulate matter of mainstream tobacco condensate [26]. 

Clove yields three types of volatile oil - oil extracted from the leaves, the stem and the buds. These oils differ considerably in yield and quality. The yield and composition of the oil obtained are influenced by its origin, season, variety and quality of raw material, maturity at harvest, pre- and post-distillation treatments and method of distillation. The chief component of the oil is eugenol. 

Method 1 Extraction of eugenol from store bought cloves... 

Another Lauraceae is Laurus nobilis (laurel). The EO obtained from the leaves of wild grown shrubs is characterized by a very high content of eugenol. The biological activities, especially the antioxidative properties, of the extract were studied in different in vitro test methods. The scavenging capacity in the DPPH assay yielded an IC50 value of 0.113 mg/mL. Also the P-carotene bleaching test of the nonpolar fractions was able to protect the lipids from oxidation. After an incubation... 

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