EGTA, ethylene glycol

Microtubules can be reconstituted in vitro at 37 °C from a solution that contains a physiological mixture of brain tubulin, MAPs, small amounts of guanosine 5 -triphosphate (GTP), magnesium ions, and the calcium-chelating agent EGTA [ethylene glycol-bis(2-aminoethyl ether) N, N -tetraacetic acid]. Tubulin assembly is inhibited by low temperature and by the presence of calcium ions. 

In this laboratory, we also include the metal ion chelators EDTA (ethylene diamine tetraacetic acid binds, e.g., Mg2 1 -ions) and EGTA (ethylene glycol-bis(2-aminoethyl)-Al,iV,iV/,iV/,-tetraacetic acid binds, e.g., Ca2+-ions) in our lysis buffers. These agents help prevent phosphatase action (by the metal ion-dependent phosphatase PP2C, which is not inhibited by microcystin-LR), metal (Ca2+) dependent proteinases, and protein kinases, which require divalent cations such as Mg2 1 (and, in some cases, also Ca2+). We also use a mix of proteinase inhibitors that inhibit a broad range of proteolytic enzymes, including serine and cysteine proteinases. 

CHES 3-(Cyclohexylamino)ethanesulfonic acid EGTA Ethylene glycol bis(/3-aminoethyl ether)-A,A,A, A -tetraacetic acid or its conjugate base... 

EGTA ethylene glycol bis(-aminoethyl ether) tetraacetate... 

Whey proteins have been purified on a hydroxyapatite support using EGTA (ethylene glycol bis( /3-aminoethylether)-N, N, N, N tetraacetic acid) and PGA (polyglutamic acid) as displacers.48... 

Sterile 0.1 M EGTA, pH 7.9—Dissolve 1.9 g of EGTA (ethylene glycol-bis(/3-aminoethyl ether) N, N, N, N -tetraacetic acid) in 35 ml of distilled water, titrate to pH 7.9 with 1 N NaOH, and dilute to 50 ml final volume with distilled water. Autoclave in a capped test tube (20 min at 105-110°C) and cool. 

Inhib. abbrev. DTT = dithioghreitol E64D = epoxysuccinyl-L-leucyl-amido-3-methyl-butane ethyl ester EDTA = ethylene-diamine-tetra-acetic acid EGTA = ethylen-glycol-tetra-acetic acid LHVS = morpholinurea-leucine-homophenyl-alanine-vinylsulfone-phenyl NEM = N-ethyl-maleimide PAI = plasminogen activator inhib. PMSF = phenil-methyl-sulfonil fluoride SBTl = soybean trypsin inhib. TIMP = tissue inhib. of metalloproteinases TPCK = tosyl-L-phenyl-alanyl-chloro-methane Al.so termed stephins. 

EGTA, ethylene glycol bis(P-aminoethylether)-A,A-tetraacetic acid 238... 

EGTA ethylene glycol-bis(P-aminoethyl ether)-Af JV -tetraacetic acid... 

Reagents for preparation HeLa cell-free extract PBS buffer (pH 7.4), 100 mM EGTA (ethylene glycol tetraacetic acid), 50% glycerol, 100 mM CaCl, Staphylococcus aureus nuclease solution (Boehringer Mannheim, lyophilized, resuspended to... 

Remarks EGTA= ethylene glycol bis(2-aminoethyl ether)-N,N,N, N -tetraacetic acid assignments for AH2 resonances are tentative assignments of both pairs of AH2 /2" resonances probably should be reversed (compare [86P1] for cs data on the same sequence). 

Remarks EGTA=ethylene glycol bis(2-aminoethyI ethcr)-N,N,N, N -tetraacctic acid. 

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