Ethylene diamine tetra-acetic acid extractant

Specific extractants and sequential fractionation are also widely used procedures to estimate metal or nutrient availability to plants (see, e.g., Houba et al. 1996). Several extractants can be considered, like diethylene triamine penta acetate (DTPA), ethylene diamine tetra acetic acid (EDTA), acetic acid, HN03, HC1, or other... 

Where atomic absorption spectrophotometry is possible the ammonium acetate extract can be directly analysed for Ca and Mg. The spectrophotometric standards are prepared in the ammonium acetate solution and both the standard and extracts are read against ammonium acetate as blank. If AAS is not possible the calcium and magnesium are analysed by complexometric titrations using ethylene diamine tetra acetic acid (EDTA). 

Many extractants were recommended for use as bioavailable indices based on significant correlation between quantities of metal extracted from the soils by the extractants and metal uptake by plants. The most commonly used extractants were ammonium bicarbonate-diethyl triamine penta acetic acid (AB-DTPA) (Soltanpour and Schwab, 1977 Norwell, 1984) and ammonium acetate acetic acid-ethylene diamine tetra acetic acid (AAAc-EDTA) (Lakanen and Ervio, 1971 Sillanpaa and Jansson, 1992). AB-DTPA was used successfully as an extractant for characterizing the bioavailability of both native soil metals as well as metals added to soils in sewage sludges. Some authors also reported insignificant relationships between the AB-DTPA-extracted metals and test plant metal concentrations (e.g. Haq and Miller, 1972 Rappaport et al, 1988). O Connor... 

In the extraction of protein from archaeological bone, subsamples are usually demineralized in dilute HCl, or via ethylene diamine tetra-acetic acid (EDTA) extraction, and proteins are precipitated. Where preservation... 

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