Estrogens and Feminization of Fish

Through fractionating WWTW effluents and screening those fractions with cell-based bioassays responsive to estrogens, the natural steroidal estrogens Ej and 

Organic Pollutants An Ecotoxicological Perspective, Second Edition 

In that study there was an inverse correlation between reproductive performance and severity of gonadal intersex (Jobling et al. 2002b). 

More controversially, endocrine disruption as a consequence of exposure to the herbicide atrazine (2-chloro-4-ethylamine-6-isopropylamine-s-triazine), one of the most widely used herbicides in the world, has also been hypothesized to explain various adverse biological effects in frog populations in the United States. Exposure to atrazine in the laboratory at high concentrations, far exceeding those found in the natural environment, has been reported to induce external deformities in the anuran species Rana pipiens, Rana sylvatica, and Bufo americanus (Allran and Karasov 2001). Studies by Hayes et al. have suggested that atrazine can induce hermaphroditism in amphibians at environmentally relevant concentrations (Hayes et al. 2002 Hayes et al. 2003). Laboratory studies with atrazine also indicated the herbicide 

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