Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Articles Figures Tables About

Estrogen receptors assay screening

An example of a TIE approach is that described by Desbrow et al. [7]. In this work, the endocrine disrupting activity detected in effluents of seven UK WWTPs by means of a yeast-based screening assay [52] was mainly attributed to the presence of estradiol, estrone, and ethynylestradiol. However, to assess the estrogenic activity different bioassays may be used, e.g., the yeast-based recombinant estrogen receptor-reporter assay (YES), the MCF-7 cell proliferation (E-screen), and the estrogen receptor-mediated chemically activated... [Pg.15]

Fig. 8.3 Yeast-based screen for agonists or antagonists of the human estrogen receptor. The hormone estrogen (estradiol) binds to the estrogen receptor which is expressed from a gene driven by the PGK promoter. The hormone-receptor complex binds to an estrogen-responsive element (ERE) that controls the expression of the p-galactosidase reporter gene. The assay measures the activity of the enzyme using a substrate that forms a colored product on conversion. Fig. 8.3 Yeast-based screen for agonists or antagonists of the human estrogen receptor. The hormone estrogen (estradiol) binds to the estrogen receptor which is expressed from a gene driven by the PGK promoter. The hormone-receptor complex binds to an estrogen-responsive element (ERE) that controls the expression of the p-galactosidase reporter gene. The assay measures the activity of the enzyme using a substrate that forms a colored product on conversion.
In estrogen receptor-positive human breast cancer cells (MCF-7), antiestrogenic activity of an aqueous extract of anise was observed (Kassi et al. 2004). Some estrogenic activity of anise essential oil was observed in a yeast estrogen screening assay (Tabanca et al. 2004). [Pg.658]

Kojima H, Katsura E, Takeuchi S, Niiyama K, Kobayashi K. Screening for estrogen and androgen receptor activities in 200 pesticides by in vitro reporter gene assays using Chinese hamster ovary cells. Environ Health Perspect 2005 112 524-31. [Pg.345]


See other pages where Estrogen receptors assay screening is mentioned: [Pg.277]    [Pg.91]    [Pg.156]    [Pg.927]    [Pg.98]    [Pg.93]    [Pg.229]    [Pg.248]    [Pg.136]    [Pg.604]    [Pg.414]    [Pg.131]    [Pg.379]    [Pg.345]    [Pg.285]    [Pg.290]    [Pg.114]    [Pg.160]    [Pg.451]    [Pg.440]    [Pg.481]    [Pg.485]    [Pg.80]    [Pg.114]    [Pg.1090]    [Pg.87]    [Pg.208]    [Pg.161]    [Pg.124]    [Pg.147]    [Pg.223]    [Pg.254]    [Pg.57]    [Pg.937]    [Pg.192]    [Pg.521]    [Pg.2240]    [Pg.2537]    [Pg.134]    [Pg.15]    [Pg.458]    [Pg.60]   
See also in sourсe #XX -- [ Pg.604 ]

See also in sourсe #XX -- [ Pg.604 ]




SEARCH



Assays estrogen assay

Assays receptor

Estrogen assay

Estrogen receptor

Screening assay

© 2024 chempedia.info