Estrogen assay

ODUM J, LEFEVRE P A, TITTENSOR S, PATON D, ROUTLEDGE E J, BERESFORD N A, SUMPTER J p and ashby j, The rodent uterotrophic assay critical protocol features, studies with nonyl phenols, and comparison with a yeast estrogenic assay , Regul Toxic Pharmacol 1997 25 176-88. 

A 24-analog hydroxystilbene library 34 was constructed from the Horner-Emmons olefination of four resin-bound hydroxybenzaldehyde BBs 33 and six different benzyl phosphonate anions [32], Screening for activity in a cell-based estrogenic assay identified several analogs such as 35 with IC50 values in the 5-15 pM range. 

Major incubation volumes were prepared for each of the estrogen assays, and from each incubate 600-/xl samples were applied onto 20 minicolumns that were centrifuged together. Table VI reports the values that were measured with coefficients of variation between 2.5% and 5.4%. 

P3. Paulsen, C. A., ed., Estrogen Assays in Clinical Medicine. Univ. of Washington Press, Seattle, Washington, 1965. 

Androgenic activity is most commonly assessed in castrated, immature male rats. For hormonal assay some investigators have preferred to use random bred animals rather than homozygous animals. Some years ago Emmens (20) made an excellent case for use of random bred mice for estrogen assays. Despite this, I prefer to use homozygous animals whenever possible. This animal variable can be standardized, and our own experience with homozygous animals bred in our laboratory has been much more salutary than our experience with the random bred animals that we have purchased. 

---