Erythropoietin periodate oxidation

Fig. (25). Periodate oxidation (A) and reaction with lectins (B) of anti-erythropoietin antibodies (S), EO, E2, E8= hrs of oxidation, LI = Maackia, L2 = Sambucus.

Results obtained" from the reaction of periodate with oligosaccharide structures in erythropoietin are consistent with the proposal that antibodies directed against the recombinant hormone are anti-carbohydrate . That oligosaccharide sequences are involved in the binding of human spermatozoa to a glycoprotein of the zona pellucida, is indicated " " by a marked decrease in binding that accompanies selective periodate oxidation of terminal sialic acid residues of the glycoprotein. 

Antibodies directed against recombinant erythropoietin have been obtained from rabbits immunized with the hormone and Freunds complete adjuvant [64], Two sets of antibodies are present in the serum of rabbits that have been immunized, Fig. (3C). The results of oxidation of the erythropoietin with periodate, Fig. (25A), reaction of the antibodies with lectins of known carbohydrate specificity, Fig. (25B) and inhibition of the antibodies with the structural monosaccharide residues of the hormone, Fig. (26), have established the types of antibodies to be anti-carbohydrate antibodies and the immunodeterminants for these antibodies are oligosaccharides with the following structure N-acetyl neuraminyl a(2->3)... 

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