Eppendorf pipets

Guard column, Betasil Cig, 10 nun x 2-mm i.d. Guard column holder Eppendorf fixed-volume pipets, 0.50-mL Eppendorf fixed-volume pipets, 1.0-mL Eppendorf pipet tips, 1.0-mL... 

Eppendorf pipet A type of micropipet that delivers adjustable volumes of liquid. 

Some workers have reported loss of volatile organic Cr compounds at low temperatures, especially in tissue samples and in plants. Recent papers seem not to be troubled by this problem, probably because of the improved experimental conditions. Some workers have used nitrogen as a purge gas instead of argon. It has been shown that narrow CN bands introduce a continuum correction error at the 357.9-nm line in the presence of nitrogen. Argon should be used. Contamination of plasticware, pipets, etc. is a problem with Cr. Older Eppendorf pipets have stainless steel springs which can introduce Cr contamination. 

Micro-pipetting instruments such as the "Eppendorf or "Oxford pipettors with disposable plastic cone tips are customarily employed to dispense the liquid samples into electrothermal atomizers. Sampling problems which are associated with the use of these pipettors are among the troublesome aspects of electrothermal atomic absorption spectrometry (67,75). The plastic cone-tips are frequently contaminated with metals, and they should invariably be cleaned before use by soaking in dilute "ultra pure nitric acid, followed by multiple rinses with demineralized water which has been distilled in a quartz still. 

C.K. CHOI AND M.W. DONG Eppendorf EDOS 5222 pipetting station... 

FIGURE 4 Pipetting devices Eppendorf pipettes, Eppendorf EDOS 5222 electronic dispensing system, PerkinElmer Packard MultiProbe II EX and Tomtec Quadra 3 96-well plate liquid handling system. 

Determine the amount of protein necessary for saturation of the gold sol Pipet 0.5 ml of the dialyzed colloidal gold into Eppendorf tubes and add 0.1 ml of a dilution series of the dialyzed protein to... 

Dilute sarcolemmal vesicles from Protocol 5.3.2 to about 1 mg pro-tein/ml with Soln. A (for protein concentration determination the Lowry method is recommended). Add 1 pi of Soln. B per 100 pi SL dilution, vortex and put on ice. Pipet the probes in triplicates into Eppendorf tubes according to Table 5.6. Label the ouabain-containing tubes with those without ouabain should be signed... 

A 50-pl aliquot of plasma, control plasma, or calibration standard is pipetted into an Eppendorf vial. Add 20 pi of the internal standard mixture and 500 pi of acetonitrile. Mix on a Vortex mixer and allow deproteinization at 4°C for 15 min. Centrifuge at... 

Plasticware sterile 35-mm and 90-mm Petri dishes, autoclaved yellow and blue pipet tips, 1.5- and 0.5-mL Eppendorf tubes. 

Transfer a few beads of resin with a Pasteur pipet into an Eppendorf tube and add two drops of DIPEA followed by five drops of TNBSA solution. Inspect the beads by eye or under a microscope if possible. If the beads are colorless after 1 min, then acylation is complete and the next step can be carried out. Any trace of orange color in the beads indicates the presence of free amino groups and incomplete coupling. In these cases steps 2-5 should be repeated until a negative TNBSA test is returned. 

Standard Curve [Note The sample injection technique is the most crucial step in controlling the precision of the analysis the volume of the sample must remain constant. Rinse the p,L pipet tip (Eppendorf or equivalent) three times with either the Standard Solutions or Sample Solution before injection. Use a fresh pipet tip for each injection, and start the atomization process immediately after injecting the sample. Between injections, flush the graphite tube of any residual... 

Eppendorf Microliter Pipet (West German manufactured)... 

Eppendorf Microliter Pipet (West German manufactured) Brinkman Instruments. Inc. Cautrague Road Westbury. NY 11590 11 H... 

Water wash The band is placed in one of the pre-washed Eppendorf tubes and 300 pL of distilled/deionized water added. After 15 min the water is removed carefully using a pipet with a fine tip. This step removes the acetic acid contained in the destain solution used for storage. 

It is important to resuspend the cells efHciently in the extraction buffer, this is complicated by the released DNA, but it is usually relatively straightforward to pipet the extract into an Eppendorf tube. This shears some of the DNA. Transfer all of the solution, including the foam. In many instances, it helps extraction if the cells are resuspended in (e.g., 50 pL) culture medium, then an equal volume of double-strength extraction buffer added as soon as possible. 

In order to modify NS with 4MBA, a 10 mM solution of 4MBA in 9 1 H20/Et0H was prepared. This solution was then pipetted as a 10 pL drop on the aggregated spots described above and left at RT overnight. On the next day, the self-assembled monolayer was washed ten times with ddH20 and the slides were spun dry at 1,000 rpm for 1 min in a table top Eppendorf centrifuge. 

All standards were prepared by dilution of Alfa Inorganics Ventron primary standard solutions using acidified filtered surface Gulf Stream seawater (salinity ca. 36% ) or distilled water. Sample injections were made with Eppendorf microliter pipets with disposable plastic tips. 

Pipet 1.5 mL of the cell culture to be tested into an Eppendorf tube and centrifuge at 14,000g for 10 min in an Eppendorf centrifuge. 

Prepare 200 pM diluted ATP solution by diluting 4 pi of 40 mM ATP solution in 800 pi 0.8 U/ml diluted pyrophosphatase solution in a 1.6 ml Eppendorf tube. Mix by pipetting and then transfer 400 pi of the mixture to a fresh tube with 400 pi diluted pyrophosphatase solution for twofold serial dilutions to reach the following concentrations 200, 100, 50, 25, 12.5, 6.25, 3.13, 0 pM. Evenly distribute the content of each vial into 12 wells in a new 384-well intermediate plate, each ATP concentration occupying a column of the plate at the upper left corner. Repeat this step for ADR... 

Besides the manually operated syringes, there are electronically controlled and variable-volume motor-driven syringes available for automated repetitive deliveries. Also, you may purchase pipets with multiple syringes for simultaneous delivery, with for example, 12 or 16 channels. These are useful for delivering solutions into microwell plates used in biotechnology or chnical chemistry laboratories that process thousands of samples (Figure 2.12). You may find more information on displacement pipets from representative manufacturers, for example, www. finnpipette.com and www.eppendorf.com. 

Pipet aliquots of 50 j L of EDTA blood into four acid-washed 1500 fiL Eppendorf centrifuge tubes containing 850 nL of 0.1% Triton X-100. Flush the pipet tip 3-4 times with the Triton X-100 solution to minimize transfer error. 

Pipet into tube 1 100 of water, into tube 2 100 of standard solution 1 (2000 hqIL Pb), into tube 3 100 nL of standard solution 2 (4000 /ig/L Pb), into tube 4 100 nL of standard solution 3 (6000 ug/L Pb). The standard solutions 1 - 3 are prepared by diluting the stock solution with 0.01 M HNO3. To make a chemical blank pipet 150 nL of water and 850fiL of 0.1% Triton X-100 into an Eppendorf centrifuge tube. 

Take sufficient Dynabeads M280 precoated with secondary antibody for the number of immunoprecipitation reactions proposed in a 1.5-mL Eppendorf tube and concentrate in MPC by placing in MPC rack for 2 min, tilting rack to horizontal at 1 min to remove any beads trapped in lid. Remove supernatant with a pipet and resuspend Dynabeads in 1 mL of PBS/BSA. Repeat, washing a total of two times. 

---