Eosin protocol

Stain 4-5 pm sections using standard hematoxylin and eosin protocols (H E) or with cresyl violet/luxol fast blue (CV/LFB). H E stains protein rich cells (eosin) and counterstains nuclei (hemotoxylin). CV/LFB also stains protein,... 

In another liver foci study using Sprague-Dawley (Crl CD) rats, 1,2-dibromoethane in corn oil given by gavage was used as an initiator. Two dose regimens were used 75 mg/kg 1,2-dibromoethane at 0 and 24 hours or corn oil at 0 hours and 75 mg/kg 1,2-dibromoethane at 24 hours. Partial hepatectomies and phenobarbital in drinking water also were part of the protocol. With this system, at 16 months, 1,2-dibromoethane-exposed rats had increased numbers of foci of hepatic cellular alteration. Rats that received the two doses of 1,2-dibromoethane had increased numbers of nodules on hematoxylin and eosin-stained sections as well as increased number and size of GGT positive foci (Moslen 1984). These results indicate that 1,2-dibromoethane can act as an initiator. 

One of the most commonly used staining procedures involves hematoxylin and eosin, but it was found that the dyes interfere with either the proteins or the MALDI process. As a consequence, the quality of the mass spectra is significantly compromised [54], When five other staining protocols (Terry s Polychrome, Toluidine Blue, Nuclear Fast Red, Cresyl Violet, and Methylene Blue) were compared to a control section (unstained tissue rinsed in 70% and 100% ethanol), Cresyl Violet and Methylene Blue had the highest degrees... 

Hemotoxylin-eosin staining protocol is used to observe histology of cancer tissue of treated and non-treated animals. Other parallel methods could be followed to reach the similar outcome. 

The protocols described below illustrate (1) frozen section sample preparation, (2) hematoxylin and eosin (H E) tissue staining, and (3) automated LCM. Alternative tissue preparation methods, such as ethanol or formalin fixation with paraffin embedding, are acceptable for RNA and DNA analysis... 

In Section 2.5 we described the use of time-resolved fluorescence anisotropy for monitoring protein motion on the nanosecond timescale. For motion on much longer timescales, time-resolved phosphorescence anisotropy can be used instead. The latter technique has been employed, for example, to examine the rotational motion of membrane-boimd proteins labelled with the triplet probe eosin (57, 58). Prior to making the measurements, the protein is labelled with eosine-maleimide as described in Protocol 2. 

Brusilovskiy, A. I. Xissue staining protocol using eosin and hematoxylin solutions. U.S. Pat. Appl. Publ. US 2005202524, 2005 Chem. Abstr. 2005, 143, 282169. 

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