Enzymes identification

Duffy JE, Hay ME (2001) The ecology and evolution of marine consumer-prey interactions. In Bertness MD, Gaines SD, Hay ME (eds) Marine community ecology. Sinauer, Sunderland, pp 131-158 Duisken M, Sandner F, Blomeke B, Hollender J (2005) Metabolism of 1,8-cineole by human cytochrome P450 enzymes identification of a new hydroxylated metabolite. Biochim Biophys Acta 1722 304-311... 

Within this common core structure, modifications have been identified that provide catalases with further unique properties. The large-subunit enzymes have extensions at both the amino and carboxyl ends, the latter having a flavodoxinlike structure, a unique His-Tyr bond, a protected cysteine, and a modified heme. NADPH binding and an oxidized methionine are found in small subunit enzymes. Identification and assignment of roles to channels providing access to and egress from the deeply buried heme have recently become the focus of study. Analysis of the structure of catalase HPII of E. coli has been facilitated by the construction of more than 75 mutants (Table I). 

Guo, L., Dixon, R.A., and Paiva, N.L., Conversion of vestitone to medicarpin in alfalfa (Medicago sativa L.) is catalyzed by two independent enzymes. Identification, purification, and characterization of vestitone reductase and 7,2 -dihydroxy-4 -methoxyisoflavanol dehydratase. J. Biol Chem., 269, 22372, 1994. 

Hou, C.T. 1995. Is strain DS5 hydratase a C-10 positional specific enzyme Identification of bioconversion products from a- and y-linolenic acids by Flavobacterium sp. DS5, /. Ind. Microbio., 14, 31-34. 

Wintroub, B.U., Kaempfer, C.E., Schechter, N.M. and Proud, D. (1986a). Human lung mast cell chymotrypsin-like enzyme identification and partial characterization. J. Clin. Invest. 77, 196-201. 

The next paragraphs try to interpret frequently occurring classes of conservation patterns , meaning the set of totally invariant or at least highly conserved positions in a family alignment. 1Tie conservation patterns of enzyme families are different from those of non-catalytic proteins and can be used for enzyme identification and classification. 

The 2006 draft FDA guidance for drug interactions suggests that in vitro studies should be conducted to identify drug metabolizing enzymes if the metabolic pathway contributes more than 25% of a drug s clearance based on human in vivo data. Whenever possible, these in vitro experiments should be conducted with drug concentrations deemed appropriate by kinetic experiments. Enzyme identification experiments should be conducted under initial rate conditions, where the linearity of metabolite production rate is assumed... 

Shebley M, Jushchyshyn MI, Hollenberg PF (2006) Selective pathways for the metabolism of phencyclidine by cytochrome P450 2B enzymes identification of electrophilic metabolites, glutathione and N-acetyl cysteine adducts. Drug Metab Dispos 34 375-383... 

Tang Z, Martin MV, Guengerich FP (2009) Elucidation of functions of human cytochrome P450 enzymes identification of endogenous substrates... 

Hohne, M., Schalzle, S., Jochens, H., Rohins, K., and Bornscheuer, U. (2010) Rational assignment of key motifs for function guides in sUico enzyme identification. Nat. Chem. Biol., 6, 807-813. 

Enzyme Identification (Mannosidases) Localization of Enzyme Oligosaccharide Substrate Final Product pH Optima Cation Requirement Mol. Wt. of Protein Is it a Glyco-Protein ... 

Kemper WM, Berry KW, Merrick WC (1976) Purification and properties of rabbit reticulocyte protein synthesis initiation factors M2Balpha and M2Bbeta. J Biol Chem 251 5551-5557 Kim SC, Sprung R, Chen Y et al (2006a) Substrate and functional diversity of lysine acetylation revealed by a proteomics survey. Mol Cell 23 607-618 Kim YS, Kang KR, Wolff EC d al (2006b) Deoxyhypusine hydroxylase is a Fe(ll)-dependent, HEAT-repeat enzyme. Identification of amino acid residues critical for Fe(II) binding and catalysis [corrected]. J Biol Chem 281 13217—13225... 

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