Enzymes performance

Van Deurzen MPJ, Seelbach K, van Rantwijk F, Kragl U, Sheldon RA (1997) Chloroperox-idase use of a hydrogen peroxide-stat for controlling reactions and improving enzyme performance. Biocatal Biotransformation 15 1-16... 

If the ultimate worth of antibody catalysts is to be more than academic, then the key must be found in their programmability. Here, the capabilities of abzymes such as their promotion of disfavoured processes and selectivity for substrates and transformations for which there are no known enzymes may offer prospects more significant than the further chasing after enzyme performance. 

For understanding the interaction between a drug and an enzyme, it is important to know how do enzymes catalyse the reaction (Section 5.2.4). In their catal5dlc activity, enzymes perform two major functions ... 

Application of PSSC is not limited to existing crystal structures of proteins. It could be applied to structures derived from homology models as well. A homology model, as applied for the 11 HSD types 1 and 2 enzymes performed well in the first application of PSSC for compound library design. Furthermore, it should be noted that the concept has not been developed to make predictions on structural complementarity or the appropriate orientation of functional groups in the binding site. 

Since biotransformation involves enzymes, there are many factors which can affect enzyme performance in a positive or negative way. 

Detergent enzyme performance is often reported in the form of such dose-response curves. The performance increases dramatically at the beginning, but reaches a maximum levd at higher enzyme concentrations. The extent to which the enzyme is able to remove stains from the fabric depends on the detergent system, temperature, pH, washing time, wash load, etc. Enzyme wash performance varies between liquid and powder detergents and with the composition of the soiling (Fig. 6). 

Correlation of Enzyme Performance with Solvent Parameters... 

After presenting the criteria for performance evaluation of the three different techniques, we explain their meaning and significance. In the next section, we will comment on the appropriateness of the criteria and the reference points. This discussion relates to coverage of enzyme performance criteria in Chapter 2. Jacobsen and Finney suggest the following five criteria for comparison of performance ( Jacobsen s five criteria ) ... 

Based on these arguments, we developed the simple directed evolution strategy illustrated in Fig. 1 [2, 6], In comparison to the enzyme performing a function for which it is selected in nature, the new job is performed poorly indeed. Beneficial mutations will be identified during screening the products of... 

For example, in the case of ECB deacylase the initial investigations showed that the enzyme was most active at high pH and temperature (e.g., pH 8, 70°C), but that the product (ECB nucleus) degraded rapidly under these conditions. One solution was to perform the bioconversion at lower pH and temperature, where the product would be stable, and accept suboptimal enzyme performance (i.e., slower reaction time or more enzyme usage). 

---