Enzymatic survey

Biological free radicals occur as more or less stable intermediate products during many enzymatic reactions. Elavin and heme radicals and porphyrin molecules are relevant examples of food colorants. Extended literature surveys are available... 

In an effort to develop easy-to-use ketoreductase toolbox , we have surveyed the activity and enantioselectivity of a collection of ketoreductases (KRED) from various sources toward the reduction of a variety of ketones [90,91]. These studies served as a useful guideline for developing enzymatic processes for the production of optically pure chiral alcohols. For example, several chiral chlorohydrins of pharmaceutical importance were synthesized in both enantiomeric forms using the enzymes in this ketoreductase collection (Table 7.2) [92]. Further applications of this collection and other commercially available ketoreductases can be found in a recent review [9]. 

This report surveys new routes for the synthesis of oligosaccharides, with emphasis on enzymatic reactions, since they offer unique properties, proceeding highly regio-and stereo-selectively in water solution, and generally afford high yields. Summarized... 

Currently, only a handful of examples of unique protein carboxylate-zinc interactions are available in the Brookhaven Protein Data Bank. Each of these entries, however, displays syn coordination stereochemistry, and two are bidentate (Christianson and Alexander, 1989) (Fig. 5). Other protein structures have been reported with iyw-oriented car-boxylate-zinc interactions, but full coordinate sets are not yet available [e.g., DNA polymerase (Ollis etal., 1985) and alkaline phosphatase (Kim and Wyckoff, 1989)]. A survey of all protein-metal ion interactions reveals that jyw-carboxylate—metal ion stereochemistry is preferred (Chakrabarti, 1990a). It is been suggested that potent zinc enzyme inhibition arises from syn-oriented interactions between inhibitor carboxylates and active-site zinc ions (Christianson and Lipscomb, 1988a see also Monzingo and Matthews, 1984), and the structures of such interactions may sample the reaction coordinate for enzymatic catalysis in certain systems (Christianson and Lipscomb, 1987). 

Excellent survey of the uses of NMR, mass spectrometry, and enzymatic reagents to determine oligosaccharide structure. 

Lmoleic acid [60-33-3] - [ALKYD RESINS] (Vol 2) - [TALL OIL] (Vol 23) - [CASTOR OIL] (Vol 5) - [COSMETICS] (Vol 7) - [CARBOXYLICACIDS-SURVEY] (Vol 5) -m dimer acid feedstocks [DIMER ACIDS] (Vol 8) -m drying oils pRYING OILS] (Vol 8) -enzymatic oxidation pNZYTffiS IN ORGANIC SYNTHESIS] (Vol 9) -m fats and oils [CARBOXYLIC ACIDS - ECONOMIC ASPECTS] (Vol 5) -as radioprotective agent [RADIOPROTECTIVEAGENTS] (Vol 20) -m soybeans and other oilseeds [SOYBEANS AND OTHER OILSEEDS] (Vol 22)... 

Freudenberg realized the importance of investigating this possibility. In 1937 he found that dehydrogenation of coniferyl alcohol with ferric chloride seemed to proceed in a way comparable with that of isoeugenol, and in 1943 he started his studies on the enzymatic dehydrogenation of coniferyl alcohol. It would not be possible here to give even a brief survey of the outstanding work which he has done since then. 

In this context, some experimental results relevant to these open questions of enzymatic degradation will be presented and will be discussed from the viewpoint of cellulose chemistry, together with a summary of our recent work on thermohydrolysis and acid hydrolysis of cellulose, performed in connection with research on cellulose powder manufacture (7). After a short survey of the experimental techniques applied, this contribution will be centered on three problems (1) the interaction of chain degradation and cross-linking in thermal and thermo-hydrolytic treatments of cellulose, (2) the influence of mechanical strain... 

The first article by Nodwell and Sieber provides a general introduction, surveying analytical platforms for ABPP, enzymatic classes addressable by ABPP probes, and biological applications of ABPP. 

Tsai reviewed the separation methods used in the determination of choline and acetylcholine [10]. This review surveyed the array of analytical techniques that have been adopted for the measurement of acetylcholine or its main precursor/metabolite (choline), ranging from simple (bioassay, radio enzymatic assay, gas chromatography-flame ionization detection, gas chromatography-mass spectrometry, high... 

Survey of Enzymatic Activities Assayed by the HPLC Method... 

SURVEY OF ENZYMATIC ACTIVITIES ASSAYED BY THE HPLC METHOD... 

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