Enhanced yellow fluorescent protein

DOTMA E. coli E EBV ECFP ECV EGFP ELISA EYFP FACS FdG FH2 FH4 FK506 FLP propane-aminium-trifluoracetate 7V-[2,3-(dioleyloxy) propyl]-/V,/V,/V-trimethyl ammonium chloride Escherichia coli erythromycine operon/repressor Epstein-Barr virus enhanced cyan fluorescence protein extracellular viral particles enhanced green fluorescence protein enzyme-linked immunosorbent assay enhanced yellow fluorescence protein fluorescence-activated cell sorter fluorescein di- 3-D-galactopyranoside dihydrofolate tetrahydrofolate human immunophilins native recombinase isolated from the 2pm plasmid from Saccharomyces cerevisiae... 

Enhanced yellow fluorescent protein (EYFP). One of the brightest fluorescent proteins. 

Bruns et al. pioneered self-reporting materials based on the mechanical perturbation of proteins within polymeric matrices/ " The protein cage thermosome (THS), a chaperonin from the archaea Thermoplasma acid-ophilum, is composed of sixteen protein subunits that form two hemispheres with the ability to enclose macromolecular guests (Figure 11.13)/ THS is approx. 16 nm in diameter. The hemispheres of chaperonins are connected through a mechanically weak plane, so that the two halves of these protein cages can be separated from each other by mechanical forces, as demonstrated in AFM experiments.THS was turned into a sensor for mechanical deformation by covalently linking two proteins, enhanced cyan fluorescent protein (eCFP) and enhanced yellow fluorescent protein (eYFP), into the... 

EBFP Enhanced Blue Fluorescent Protein ECFP Enhanced Cyan Fluorescent Protein EGFP Enhanced Green Fluorescent Protein EYFP Enhanced Yellow Fluorescent Protein. 

Fig. 2 List of some of the most widely employed fluorochromes in FM with indication of their peaks of excitation and emission. On the right are indicated the main lines of emission of a mercury lamp (ML) and four common laser sources. According to the wavelength of emitted light, lasers can be indicated as UV lasers (350 nm), violet lasers (407 nm), Wue lasers (488 nm), and ref/lasers (635 nm). 7-A4D7-aminoactinomycin 0, APC allophycocyanin, CFP cyan fluorescent protein, Em emission (nm). Ex excitation (nm), EYFP enhanced yellow fluorescent protein, FAf fluorescence microscopy, F/ Pfluorescent reporter protein, 6FF green fluorescent protein, IMF immunofluorescence, mito mitochondrion/mitochondrial, ML mercury lamp, RFP red fluorescent protein, variant of YFP, KFFyellowfluorescentprotein...

Borst JW, Flink MA, van Hoek A, Visser AJWG (2005) Effects of refractive index and viscosity on fluorescence and anisotropy decays of enhanced cyan and yellow fluorescent proteins. JFluoresc 15 153-160... 

Recently, we reported that the yellow BL production is facilitated by a supply of 02to the 02-limited culture and that the enhanced yellow BL adversely falls to the original level after halting the forced aeration. Such a BL modulation can be regarded as a reversible response to the variation of O2 concentration. It is postulated i) that the reversible BL color change is associated with the activity of the accessory yellow fluorescent protein (YFP) and ii) that the YFP activity is governed by the redox state of the respiratory protein complexes within the cell membrane. 

FITC+FAM (fluorescein derivatives). ECFP, EGFP, EYFP (enhanced—cyan, green and yellow fluorescent protein, respectively, Clontech Laboratories, Inc., USA). Bodipy is a trademark of Invitrogen Ltd., UK. Attoxxxis a trademark of ATID-TEC GmbH, Gennany. A A S, and A J), A are the one- and two-photon absorption and emission maximum, respectively. QY is the fluorescence quantum yield, SS the Stokes shift, e the molar extinction coefficient and Tf the fluorescence lifetime. Further information regarding two-photon measurements can be found In Chapter 3. 

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