Endothelial cell interactions preparation

Kim and Rajagopalan (2010) prepared chitosan/HA multilayer scaffolds to mimic the space of Disse of liver and to preserve or enhance liver-specific behaviors of hepatocytes and liver sinusoidal endothelial cells (LSECs). Hepatocytes and LSECs cultured in the 3D hepatic model scaffold exhibited several key phenotypic characteristics for 12 days due to the enhanced heterotypic cell-cell interactions. Further, hepatocytes and LSECs on the chitosan/HA scaffolds increased CYPlAl/2 and CYP3A activity while albumin production increased threefold to sixfold. 

Since cell adhesion depends on the presence of certain proteins on a surface, protein density gradients have also been prepared and tested. Bovine serum albumin (BSA) is known to dexnease cell adhesion, while other proteins, for example fibronectin, enhance the interaction. A fibronectin concentration gradient saturated with BSA leads to the selective adhesion of fibroblasts to the fibronectin-coated r ons (Fig. 14) and to endothelial... 

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