Endothelial cell interactions Bovine aortic

Garcia-Cardena reported in 1996 that the tyrosine phosphorylation of eNOS in bovine aortic endothelial cells (BAEC) resulted in a decrease in enzyme activity whilst immunoprecipitation of caveolin-1, the predominant isoform in endothelial cells, resulted in co-immunoprecipitation of tyrosine phosphorylated eNOS [17]. Michel et al. (1997) further reported that eNOS, also in BAEC, was co-immunoprecipitaed by caveolin-1 antibodies whilst in rat myocytes eNOS was associated with caveolin-3, the cardiac muscle specific type, confirming that eNOS interaction with caveolin is not tissue specific [18]. Immunofluoresence studies in our group have shown that eNOS associates with caveolin-1 in human umbilical vein endothelial cells (HUVEC), and this interaction is abolished by bradykinin (Wyatt, Pedley Mann, 2001 unpublished data) (Figure 3). 

Chen, C. Y, Barron, J. A., and Ringeisen, B. R. 2006. Cell patterning without chemical surface modification Cell-cell interactions between printed bovine aortic endothelial cells (BAEC) on a homogeneous cell-adherent hydrogel. Appl SurfSci, 252,8641-5. 

Fig. 2 Peptide ink. This type of peptide has three distinct segments a functional segment where it interacts with other proteins and cells a linker segment that is either flexible or stiff and sets the distance from the surface, and an anchor for covalent attachment to the surface. These peptides can be used as ink for an inkjet printer to directly print on a surface, instantly creating any arbitrary pattern, as shown here. Bovine aortic endothelial cells were confined to the patterns of squares connected with linear tracks. The patterns were made with an oxygen gas treated PDMS stamp to increase the surface hydrophilicity to facilitate EG6SH wetting...

---