Embryonic stem cell gene targeting

Doetschman, T., Gregg, R G., Maeda, N., Hooper, M. L., Melton, D. W., Thompson, S., et al. (1987) Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330, 576-578. 

Poueymirou, W. T., Auerbach, W., Fren-dewey, D., Hickey, J. F., Escaravage, J. M., Esau, L., et al. (2007) F0 generation mice fully derived from gene-targeted embryonic stem cells allowing immediate phenotypic analyses. Nat Biotechnol 25, 91-99. 

Koentgen, F. and Stewart, C.L. (1993) Simple screening procedure to detect gene targeting events in embryonic stem cells. Methods Enzymol. 225, 878-890. 

Irradiated embryonic fibroblast feeder cell layer to be used for growing the embryonic stem cell line G418 resistant embryonic fibroblast cells are derived from embryos that carry a targeted disruption such as the 3-2 microglobulin gene (9). 

Figure 2.13. Procedure for making nullizygous mice using embryonic stem cells. (Adapted from Sedivy, J. M., and Joyner, A. L. Gene Targeting, Oxford University Press, New York, 1994.)...

Doetschman, T.C. (1991). Gene targeting in embryonic stem cells. In Transgenic Animals. N.L.First and F.P.Haseltine, eds. (New York Bntterworth-Heinemann), pp. 89 I0I. 

Hollnagel, A., Oehlmann, V., Heymer, J., Ruther, U., Nordheim, A. 1999. Id genes are direct targets of bone morphogenetic protein induction in embryonic stem cells. J. Biol. Chem. 274, 19838-19845. 

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