Electrophoresis Tiselius moving-boundary

A Tiselius. Moving boundary method of studying the electrophoresis of proteins. Nova Acta Reg Soc Uppsal Ser IV 7 1-107, 1930. 

Figure 7.3.4 Tiselius moving boundary electrophoresis apparatus. [After Shaw, D.J. 1980. Introduction to Colloid and Surface Chemistry, 3rd edn. London Butterworths. With permission.]...

It was not until the work of Tiselius in the 1930s that the potential of electrophoresis as a biochemical tool was realized. Tiselius developed the use of electrophoresis for separating proteins in suspension on the basis of their charge. He optimized the geometry and temperature of the system in an apparatus known as the Tiselius moving boundary system. Detection of the separated components was carried out by detecting concentration changes in optical refraction. 

The origins, principles, methods, and modes of capillary electrophoresis (CE) are discussed. Massive application of electrophoresis methods started after Tiselius s moving boundary method that was optimized by the use of paper or a gel as a semiconducting medium. The applications of paper and gel electrophoresis were situated mostly in the biochemical environment for the analysis of proteins, amino... 

With the study of the migration of hydrogenium ions (H ) in a phenolphthalein gel by Lodge in 1886 and the description of the migration of ions in saline solutions by Kohlraush in 1897, a basis was set for the development of a new separation technique that we know today as electrophoresis. Indeed, several authors applied the concepts introduced by Lodge and Kohlraush in their methods and when Arne Tiselius reported the separation of different serum proteins in 1937, the approach called electrophoresis was recognized as a potential analytical technique. Tiselius received the Nobel Prize in Chemistry for the introduction of the method called moving boundary electrophoresis. ... 

FIG. 12.11 Schematic illustration of a Tiselius-type moving boundary electrophoresis apparatus. 

A. Tiselius, The moving boundary method of studying the electrophoresis of proteins, Ph.D. Thesis, Nova acta regiae societatis scientiarum, Ser. IV, Vol. 17, No. 4, Uppsala, Sweden Almqvist Wiksell (1930), 1-107. 

Aug. 10,1902, Stockholm, Sweden - Oct. 29,1971, Uppsala, Sweden) Tiselius studied chemistry in Uppsala and joined the laboratory of -> Svedberg in 1925. In 1930 he received the Ph.D. for his thesis entitled The moving-boundary method of studying the electrophoresis of proteins . In 1937, a research professorship was established for Tiselius. His fundamental work in developing -> electrophoresis was encouraged and supported by Svedberg. In 1948 he was awarded the Nobel Prize in Chemistry for his achievements in electrophoresis and adsorption analysis. 

Electrophoresis experiments in glass tubes were reported as early as in the nineteenth century, but the first real breakthrough occurred in the first half of the twentieth century when the Swedish chemist Arne Tiselius applied free-solution electrophoresis—i.e moving boundary—to serum protein analysis, for which he later received the 1937 Nobel Prize [2], In less than two decades, just after the striking scientific discovery of the double-helical structure of DNA by Watson and Crick in 1953 [3] and the following unveiling of the genetic code, electrophoresis became a standard and indispensable tool in the field of modern... 

The moving boundary method for investigating electrophoresis has, however, been improved by Tiselius to the extent that it has become a powerful tool of investigation, particularly of proteins. Tiselius 10 early work is outlined in his dissertation which includes a valuable discussion of the conditions governing the movement of a boundary. A particularly important experimental detail is that the composition of the solution in which the colloid is suspended should be, as nearly as possible, of the same composition as the solution with which it is in contact at the boundary, otherwise boundary anomalies will be present. These will cause the particles of colloid to move abnormally fast or slow, and may even give rise to additional boundaries. 

Arne Tiselius, "The Moving Boundary Method of Studying the Electrophoresis of PrO-... 

Moving Boundary Electrophoresis An indirect electrophoresis technique for particles too small to be viewed. This principle is used in the Tiselius apparatus. Here a colloidal dispersion is placed in the bottom of a U-tube, the upper arms of which are filled with a less dense liquid that both provides the boundaries and makes the connections to the electrodes. Under an applied electric field the motions of the ascending and descending boundaries are measured. 

Tiselius Apparatus An apparatus for the determination of electrophoretic mobilities. See Moving Boundary Electrophoresis. 

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