Electron paramagnetic resonance properties

Verfiirth K, AJ Pierik, C Leutwein, S Zorn, 1 Heider (2004) Substrate specificities and electron paramagnetic resonance properties of benzylsuccinate synthesis in anaerobic toluene and m-xylene metabolism. Arch Microbiol 181 155-162. 

The possibility of using the electron paramagnetic resonance properties of Gd3+ to probe its environment in and interactions with biological molecules has previously received little attention in the literature (40). However, the possibility exists that Gd2+ will be a sensitive EPR probe for characterizing macromolecular biological systems such as the Ca2+-ATPase. The EPR spectra of Gd3+, which has S = 7/2. in neutral water and in two different buffers are shown in Figure 13A. The linewidths were found to be independent of pH over the usable range of these buffers and independent of temperature between 4 and 30°C, The spectrum of Gd2+ in neutral water is centered around 3248 G, with a linewidth of 530 G. As shown, Gd3+ in Pipes buffer, but not in Tes buffer, yielded a spectrum similar to that of the aqueous Gd2+ solution. On this basis, all of our Gd3+ EPR and NMR studies... 

JL Zimmermann and AW Rutherford (1986) Electron paramagnetic resonance properties of the S2-state of the oxygen-evolving complex of photosystem II. Biochemistry 25 4609-4615... 

Lieberman RA, Sands RH, Fee JA. 1982. A study of the electron paramagnetic resonance properties of single monocbnic crystals of bovine superoxide dismutase. J Biol Chem 257 336-344. 

Other magnetic measurements of catalysts include electron paramagnetic resonance and magnetic susceptibility. Although those are not as common as NMR, they can be used to look at the properties of paramagnetic and ferromagnetic samples. Examples of these applications can be found in the literature [87. 

Electron spin resonance (or electron paramagnetic resonance) is now a well-established analytical technique, which also offers a unique probe into the details of molecular structure. The energy levels involved are very close together and reflect essentially the properties of a single electronic state split by a small perturbation. 

Kochelaev BI, Teitel baum GB (2005) Nanoscale Properties of Superconducting Cuprates Probed by the Electron Paramagnetic Resonance 114 205-266 Kochi JK, see RosokhaSV (2007) 126 137-160 Kohler J, see Deng (2005) 114 103-141 van Koningsbruggen, see Giitlich P (2004) 107 27-76... 

ELECTROCHEMICAL PROPERTIES AND ELECTRON PARAMAGNETIC RESONANCE (EPR)-SPECTRA OF NITRONE RADICAL IONS... 

Electron paramagnetic resonance (EPR) and NMR spectroscopy are quite similar in their basic principles and in experimental techniques. They detect different phenomena and thus yield different information. The major use of EPR spectroscopy is in the detection of free radicals which are uniquely characterised by their magnetic moment that arises from the presence of an unpaired electron. Measurement of a magnetic property of a material containing free radicals, like its magnetic susceptibility, provides the concentration of free radicals, but it lacks sensitivity and cannot reveal the structure of the radicals. Electron paramagnetic resonance spectroscopy is essentially free from these defects. 

Based on our current understanding of ribosomal protein synthesis, several strategies have been developed to incorporate amino acids other than the 20 standard proteinogenic amino acids into a peptide using the ribosomal machinery . This allows for the design of peptides with novel properties. On the one hand, such a system can be used to synthesize nonstandard peptides that are important pharmaceuticals. In nature, such peptides are produced by nonribosomal peptide synthetases, which operate in complex pathways. On the other hand, non-natural residues are a useful tool in biochemistry and biophysics to study proteins. For example, incorporation of non-natural residues by the ribosome allows for site-specific labeling of proteins with spin labels for electron paramagnetic resonance spectroscopy, with... 

Grande HJ, Dunham WR, Averill B, et al. 1983. Electron paramagnetic resonance and other properties of hydrogenases isolated from Desulfovibrio vulgaris (strain Hildenborough) and Megasphaera elsdenii. Eur J Biochem 136 201-7. 

Before the availability of a high-resolution structure of P. aeruginosa CCP, the properties and environments of the two hemes had been probed using a range of solution spectroscopies. These include electron paramagnetic resonance (EPR) (51, 57, 58, 61), resonance Raman (59), circular dichroism (CD) 71, 72), MCD 58, 61, 73, 74). Until the demonstration by Ellfolk and colleagues that it is the mixed-valence form of the... 

Nanoscale Properties of Superconducting Cuprates Probed by the Electron Paramagnetic Resonance... 

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