Spectroscopy solution

Before the availability of a high-resolution structure of P. aeruginosa CCP, the properties and environments of the two hemes had been probed using a range of solution spectroscopies. These include electron paramagnetic resonance (EPR) (51, 57, 58, 61), resonance Raman (59), circular dichroism (CD) 71, 72), MCD 58, 61, 73, 74). Until the demonstration by Ellfolk and colleagues that it is the mixed-valence form of the... 

Crystal and Solution Spectroscopy of Einsteinium, W.T. Camall, Proceedings of the Symposium Commemorating the 25th Anniversary of Elements 99 and 100, Lawrence Berkeley Laboratory, January 23, 1978, Lawrence Berkeley Laboratory, Report LBL-7701 (April 1979), pp. 73-79. 

We have not discussed quantitative hydrogen bond energies and how these are measured and calculated. Neither have we discussed in any detail the interpretation of the many facets of molecular and solution spectroscopy. While these are important for analysis of the dynamical properties of biological molecules, they are not methodologies that provide the quantitative information relating to the static three-dimensional structure that is the central focus of this monograph. 

Many spectrometers are equipped with facilities to monitor and regulate the temperature within a probe head. Usually the sensor takes the form of a thermocouple whose tip is placed close to the sample in the gas flow used to provide temperature regulation. However, the readings provided by these systems may not reflect the true temperature of the sample unless they have been subject to appropriate calibration. One approach to such calibration is to measure a specific NMR parameter that has a known temperature dependence to provide a more direct reading of sample temperature. Whilst numerous possibilities have been proposed as reference materials [41], two have become accepted as the standard temperature calibration samples for solution spectroscopy. These are methanol for the range 175-310 K and 1,2-ethanediol (ethylene glycol) for 300-400 K. 

Open-chain carbohydrate derivatives are ideal objects for conformational analysis by H -NMR solution spectroscopy. The OH-proton signals are well separated from CH-methine proton signals, and decoupling experiments allow easy signal assignments. Polar carbohydrate derivatives and the carbohydrates themselves are soluble in dimethyl sulfoxide (DMSO), which destroys all inter-... 

Baker JK (1992) Nuclear Overhauser Effect Spectroscopy (Noesy) and Dihedral Angle Measurements in the Determination of the Conformation of Taxol in Solution. Spectroscopy Letters 25 31... 

Requirements for atomic absorption were shown in Figure 20-1. Principal differences between atomic and solution spectroscopy lie in the light source, the sample container (the flame, furnace, or plasma), and the need to subtract background emission from the observed signal. 

NMR spectroscopy has been extremely valuable in determining the structure of the polyazines, primarily in the undoped state. Because of the low solubility of all the polyazines except PMPAZ, very little solution spectroscopy has been reported. However, solid-state C and N NMR spectra have been measured for most of the polyazines that have been synthesized. Comparison of the polymer spectra to the spectra of model... 

Porumb H (1978) The solution spectroscopy of drugs and the drug-nucleic acid interactions. 

The tendency of solute molecules to keep their hydration shell, the roughness of the liquid surface and its dynamic fluctuations are not only important for understanding solute spectroscopy, thermodynamic, and relaxation phenomena, but also, as we show below, they play a major role in elucidating the surface effect on chemical reactivity. 

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